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Practical Column Chromatography

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 59))

Abstract

Many of the most powerful methods of protein fractionation involve the use of column chromatography. Earlier chapters in this volume describe specific chromatographic techniques, but the focus is mainly on the particular application rather than on the generalities of how to do column chromatography in practice. This final chapter is intended to fill in some of the practical gaps in terms of what materials and equipment are needed and how they should be used.

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References

  1. Bock, R. M. and Ling, N.-S (1954) Devices for gradient elution In chromatography. Anal Chem. 26, 1543–1546.

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  2. MacGregor, S. E. and Walker, J. M. (1994) A microtiter plate assay for the detection of Inhibitors of the Na+,K+-ATPase. Appl. Biochem. Biotechnol. 49,135–141.

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© 1996 Humana Press Inc.

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Doonan, S. (1996). Practical Column Chromatography. In: Doonan, S. (eds) Protein Purification Protocols. Methods in Molecular Biology™, vol 59. Humana Press. https://doi.org/10.1385/0-89603-336-8:381

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  • DOI: https://doi.org/10.1385/0-89603-336-8:381

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-336-8

  • Online ISBN: 978-1-59259-545-7

  • eBook Packages: Springer Protocols

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