Abstract
Cell culture is one of the major tools of cell biologists. It has also become an integral part of the daily routine of most oncology laboratories for the purpose of karyotyping, chemoresistance testing, or basic research. It provides investigators with an opportunity to investigate many cellular parameters and interactions in an in vitro system in which the experimental conditions can be controlled and repeated With many tissues, either human or animal, the problems of cell culture are cell attachment and initial survival. Particularly the primary cultures derived from tumor specimens are a problem in many laboratories. Apart from modifications in the composition of tissue-culture plastic materials, other approaches have been used to get around this problem, such as coating of tissue-culture dishes with attachment enhancers, such as polyamino acids (1), fibronectin (2), laminin (3), and collagen (4) Since it was known that endothelial cells are capable of producing a basement membrane even in vitro, bovine cornea1 endothelial basement membrane was explored by Gospodarowicz et al. for its role in regeneration and nonregeneration of cornea1 endothelium in different species This bovine cornea1 extracellular matrix (bECM) was found useful in the cell culture of a wide range of different cells (5, 6), and bECM as well as other ECMs were employed in the cell biology of tumor cells derived from mammary carcinomas (7), urological tumors (8), and different kinds of pituitary adenomas (9, 10), as well as CNS tumors (11), which is the topic of this chapter.
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References
Bottenstein, J. E., and Sato, G. H., (1980) Fibronectin and polylysine requirement for proliferation of neuroblastoma cells in defined medium Exp Cell Res 129, 361–366
Terranova, V. P., Aumalley, M., Sultan, L. H, Martin, G. R., and Kleinman, H. K., (1986) Regulation of cell attachment and cell number by fibronectin and laminin J Cell Physiol 127, 473–479
Couchmann, J. R., Hook, M., Rees, D., and Timpl, R., (1983) Adhesion, growth and matrix production by fibroblasts on laminin substrates J Cell Biol 96, 177–183
Varani, J., Carey, T. E., Fligiel, S. E. G., McKeever, P. E., and Dixit, V., (1987) Tumor type-specific differences in cell-substrate adhesion among human tumor cell lines Int J Cancer 39, 397–403
Gospodarowicz, D., Vlodavsky, I., and Savlon, N., (1981) The role of fibroblastgrowth factor and the extracellular matrix in the control of proliferation and differentiation of cornea1 endothelial cells Vison Res 21, 87–103
Gospodarowicz, D., Cohen, D., and Fujii, D. K., (1982) Regulation of cell growth by the basal lamina and plasma factors relevance to embryonic control of cell proliferation and differentiation, in Cold Spring Harbor Conference on Cell Proliferation., vol 9 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. pp 95–124
Lichtner, R. B., Belloni, P. N., and Nicolson, G. L., (1989) Differential adhesion of metastatic rat mammary carcinoma cells to organ-derived microvessel endothelial cells and subendothelial matrix Exp Cell Biol 57, 146–152
Pavelic, K., Bulbul, M. A., Slocum, H. K., Pavelic, Z. P., Rustum, Y. M., Nicdbala, M. J., and Bernacki, R. J., (1986) Growth of human urological tumors on extracellular matrix as a model for the in vitro cultivation of primary tumor explants Cancer Res 46, 3653–3662
Westphal, M., Jaquet, P., and Wilson, C. B., (1986) Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum free conditions Actu Neuropathol 71, 142–149
Westphal, M., Hahn, H., and Ludecke, D. K., (1987) Culture of dispersed cells from human pituitary adenomas from acromegalic patients on extracellular matrix, in Growth Hormone, Growth Factors and Acromegaly (Ludecke, D. K., and Tolls, G., eds), Raven, New York, pp 125–133.
Westphal, M., Hansel, M., Brunken, M., Konig, A., Koppen, J. A., and Herrman, H. D., (1987) Initiation of primary cell cultures from human intracranial tumors on extracellular matrix from bovine cornea1 endothelial cells Exp Cell Biol 55, 152–163
Hynes R. O., (1992) Integrins versatility, modulation and signalling in cell adhesion Cell 69, 1l–25
Alanko, T., Tienan, J., Lehtonen, E., and Saksela, O., (1994) Development of FGF-dependency in human embryonic carcinoma cells after retmoic acid-induced differentiation Dev Biol 161, 141–153
Adams, J. C., and Watt, F. M., (1993) Regulation of development and differentiation by extracellular matrix. Development 117, 1183–1198
Vlodavsky, Z., Lui, G. M., and Gospodarowicz, D. J., (1980) Morphological appearance, growth behaviour and migratory activity of human tumor cells maintained on extracellular matrix versus plastic Cell 19, 607–616
Payne, H. R., and Lemmon, V., (I993) Glial cells of the O-2A lineage bind preferentially to N-cadherin and develop distinct morphologies Dev Biol 159, 595–607
Rutka, J. T., (1986) Effects of extracellular matrix proteins on the growth and differentiation of an anaplastic glioma cell line. Can J Neural Sci 13, 301–306
Westphal, M., Hansel, M., Nausch, H., Rohde, E., and Herrmann, H. D., (1990) Culture of human brain tumors on an extracellular matrix derived from bovine cornea1 endothelial cells and cultured human glioma cells, in Methods in Molecular Biology, vol 5, Animal Cell Culture (Pollard, J. W., and Walker, J. M., eds ), Humana Press, Clifton, NJ, pp 113–131
Weiner, R. I., Bethea, C. L., Jaquet, P., Ramsdell, J. S., and Gospodarowicz, D. J., (1983) Culture of dispersed anterior pituitary cells on extracellular matrix Methods in Enzymol 103, 287–294
Gospodarowicz, D., Cheng, J., Lui, G. M., Baird, A., and Bohlen, P., (1984) Isolation of brain fibroblast growth factor by heparin sepharose affinity chromatography identity with pituitary fibroblast growth factor Proc Natl Acad Sci USA 81, 6963–6967
Westphal, M., Hansel, M., Hamel, W., Kunzmann, R., and Holzel, F., (1994) Karyotype analysis of 20 human glioma cell lines Acta Neurochir 126, 17–26
Cardwell, M. C., and Rome, L. H., (1988) Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix J Cell Biol 107, 1541–1549.
Liotta, L. A., and Stetler-Stevenson, W. G., (1991) Tumor invasion and metastasis an imbalance of positive and negative regulation Cancer Res 51, 5054–5059
Shapiro, J. R., (1986) Biology of gliomas chromosomes, growth factors and oncogenes Sem Oncol 13, 4–15
Giese, A., Rief, M., and Berens, M., (1994) Determinants of human glioma cell migration Cancer Res 54, 3887–3904
Mucke, L., and Rockenstein, E. M., (1993) Prolonged delivery of transgene prod ucts to specific brain regions by migratory astrocyte grafts Transgene 1, 3–9
Westphal, M., Stavrou, D., Nausch, H., Valdueza, J. M., and Herrmann, H.-D., (1994) Human neurocytoma cells in culture show characteristics of astroglial differentiation J Neurosci Res 38, 698–704.
Vlodavsky, I., Folkman, J., Sullivan, R., Fridman, R., Ishai-Michaeli, R., Sasse, J., and Klagsbrun, M., (1987) Endothelial cell derived basic fibroblast growth factor synthesis and deposition into subendothelial extracellular matrix Proc Natl Acad Sci USA 84, 2292–2296
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© 1996 Humana Press Inc., Totowa, NJ
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Westphal, M., Nausch, H., Zirkel, D. (1996). Cell Culture of Human Brain Tumors on Extracellular Matrices. In: Jones, G.E. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine, vol 2. Humana Press. https://doi.org/10.1385/0-89603-335-X:81
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DOI: https://doi.org/10.1385/0-89603-335-X:81
Publisher Name: Humana Press
Print ISBN: 978-0-89603-335-1
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