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Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture

A Comparative Study

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 2))

Abstract

Uterine contractility and cervical tonicity change throughout the menstrual cycle and pregnancy in response to modifications in hormonal environment and tissue receptivity to hormones. The uterine wall consists of a smooth muscle (myometrium) organized into three layers. the inner and outer layers, mainly composed of smooth muscle cells, and the richly vascularized intermediate layer The musculature is thick in the corpus uteri and vanishes at the level of the corpus/cervix Junction. The cervix itself is formed mainly by connective tissue.

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References

  1. Laguens, R and Lagrntta, J (1964) Fine structure of human uterine muscle in pregnancy Am J Obstet Gynecol 89, 1040–1048

    PubMed  CAS  Google Scholar 

  2. Cavaillé, F, Janmot, C, Roper S, and D’Albis A (1986) Isoforms of myosm and actin in human, monkey and rat myometrinm Comparison of pregnant and non-pregnant uterus proteins. Eur J Biochem 160, 507–513

    Article  PubMed  Google Scholar 

  3. Tabb, T, Thilander, G, Grover, A, Hertzberg, E., and Garfield, R (1992) An immunochemical and immunocytologic study of the increase in myometrial gap junctions (and connexin 43) in rats and humans during pregnancy Am J Obstet Gynecol 167, 559–567

    PubMed  CAS  Google Scholar 

  4. Wray, S (1993) Uterine contraction and physiological mechanisms of modulation Am J Physiol 264, Cl–C18

    Google Scholar 

  5. Uldbjerg, N, Ekman, G, Malmstrom, A, Olsson, K., and Ulmsten, U (1983) Ripening of the human cervix related to changes in collagen, glycosaminoglycans, and collagenolytic activity Am J Obstet Gynecol 147, 662–666

    PubMed  CAS  Google Scholar 

  6. Cabrol, D, Dallot, E, Cédard, L, and Sureau, C (1985) Pregnancy-related changes in the distribution of glycosamimoglycans in the cervix and the corpus of the human uterus Eur J Obstet Gynecol Reprod. Bwl 20, 289–295

    Article  CAS  Google Scholar 

  7. Chen, L, Lindner, H R, and Lancet, M (1973) Mitogenic action of estradiol-17β on human myometrial and endometrial cells in long-term tissue cultures J Endocrinol 59, 87–97

    Article  PubMed  CAS  Google Scholar 

  8. Casey, M L., MacDonald, P C, Mitchell, M D, and Snyder, J M (1984) Maintenance and characterization of human myometrial smooth muscle cells in monolayer culture In Vztro 20, 396–403

    CAS  Google Scholar 

  9. Kawaguchi, K., Fujii, S, Konishi, I, Okamura, H, and Mori, T (1985) Ultrastructural study of cultured smooth muscle cells from uterine leiomyoma and myometrium under the influence of sex steroids. Gynecol Oncol 21, 32–41

    Article  PubMed  CAS  Google Scholar 

  10. Kornyei J L, Lei, Z M, and Rao, C V. (1993) Human myometrial smooth muscle cells are novel targets of direct regulation by human chorionic gonadotropin Blol Reprod 49, 1149–1157

    Article  CAS  Google Scholar 

  11. Heluy, V., Breuiller-Fouché, M, Cavaille, F, Fournier, T, and Ferre, F (1995) Characterization of a type A endothelin receptors in cultured human myometrial cells Am J Physiol 268, E825–E831

    PubMed  CAS  Google Scholar 

  12. Gabbiani, G, Ryan, G B, and Majno, G (1971) Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction Experientia 27, 549

    Article  PubMed  CAS  Google Scholar 

  13. Skalli, O, Schurch, W, Seemayer, T, Lagace, R., Montandon, D, Pittet, B, and Gabbiani, G (1989) Myofibroblasts from diverse pathologic settings are hetero-geneous in their content of actin isoforms and intermediate filament proteins Lab Invest 60, 275–285.

    PubMed  CAS  Google Scholar 

  14. Eyden, B P, Ponting, J, Davies, H, Bartley, C, and Torgersen, E (1994) Defining the myofibroblast normal tissues, with special reference to the stromal cells of Wharton’s Jelly in human umbilical cord J Submicrosc Cytol Path01 26, 347–355.

    CAS  Google Scholar 

  15. Wiqvist, N, Linblom, B, Wikland, M., and Wilhelmsson, L (1983) Prostaglandins and uterine contractility Acta Obstet Gynecol Stand Suppl. 113, 23–29.

    Google Scholar 

  16. Bryman, I, Sahni, S, Norstrom, A, and Linblom, B (1984) Influence of prostag-landims on contractility of the isolated human cervical muscle Obstet Gynecol 63, 283–284

    Google Scholar 

  17. Cavaillé, F, Fournier, T., Dallot, E, Dhellemmes, C, and Ferré, F (1995) Myosin heavy chain isoform expression in human myometrium, presence of an embryonic nonmuscle isoform in leiomyomas and in cultured cells Cell Motil Cytoskel 30, 183–193

    Article  Google Scholar 

  18. Negishi, M, Ito, S, and Hayaishi, O (1989) Prostaglandin E receptors in bovine adrenal medulla are coupled to adenylated cyclase via Gi and to phosphomositiole metabolism in a pertussis toxin-insensitive manner J Biol Chem 264, 3916–3923

    PubMed  CAS  Google Scholar 

  19. Chamley-Campbell, J, Campbell, G. R, and Ross, R (1979) The smooth muscle cell in culture Physiol Rev 59, 1–61

    PubMed  CAS  Google Scholar 

  20. Osbor, M and Weber K (1986) Intermediate filament proteins. a multigene fami1y distiinguishing major cell lineages Trends Biochem Ser. 11, 469–472

    Article  Google Scholar 

  21. Skalli, O, Vandekerckhove, J., and Gabbiain, G (1987) Actin-isoform pattern as a marker of normal or pathological smooth-muscle and fibroblastic tissues Differentiation 33, 232–238

    Article  PubMed  CAS  Google Scholar 

  22. Frid, M. G, Printseva, O. Y, Chiavegato, A, Faggin, E, Scatena, M., Kotelianski, V E, Pauletto, P, Glukhova, M. A, and Sartore, S. (1993) Myosin heavy-chain isoform composition and distribution in developing and adult human aortic smooth muscle J Vascular Res 30, 279–292

    Article  CAS  Google Scholar 

  23. Ewoane, C. and Cavaillé, F. (1990) ATPase activity of reconstituted actomyosin from pregnant and non-pregnant human uterus, its dependence on the actin isoforms Biochem (Life Sci Adv) 9, 5–10

    Google Scholar 

  24. Somlyo, A. P (1993) Myosin isoforms in smooth muscle: how may they affect function and structure? J Muscle Res Cell Motil 14, 557–563

    Article  PubMed  CAS  Google Scholar 

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© 1996 Humana Press Inc., Totowa, NJ

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Cavaillé, F., Cabrol, D., Ferre, F. (1996). Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture. In: Jones, G.E. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine, vol 2. Humana Press. https://doi.org/10.1385/0-89603-335-X:335

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  • DOI: https://doi.org/10.1385/0-89603-335-X:335

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-335-1

  • Online ISBN: 978-1-59259-586-0

  • eBook Packages: Springer Protocols

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