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Specific PCR Primers for the Identification of Endomycorrhizal Fungi

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Species Diagnostics Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 50))

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Abstract

The sensitivity of the polymerase chain reaction (PCR) (1,2), coupled to the specificity afforded by taxon-specific primers allows the analysis of a variety of samples, like minute amounts of cultured organisms (3, 4), or even endosymbionts like the endomycorrhizal fungi present in the roots of field collected plants (58).

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References

  1. Innis, M. A., Gelfand, D. H., Sninsky, J. J., and White, T. J. (1990) PCR Protocols A Guide to Methods and Applications, Academic, San Diego, CA.

    Google Scholar 

  2. Mullis, K. B. and Faloona, F. A. (1987) Specific synthesis of DNA in vitro via a polymerase catalysed chain reaction. Methods Enzymol. 155, 335–350.

    Article  PubMed  CAS  Google Scholar 

  3. Bruns, T. D., Fogel, R., and Taylor, J. W. (1990) Amplification and sequencing of DNA from fungal herbarium specimens. Mycologia 82, 175–184.

    Article  CAS  Google Scholar 

  4. White, T. J., Brims, T., Lee, S., and Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, in PCR Protocols. A Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J, and White, T. J., eds.), Academic, San Diego, CA, pp. 315–322.

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  5. Simon, L., Bousquet, J., Lévesque, R. C., and Lalonde, M. (1993) Origin and diversification of endomycorrhizal fungi and coincidence with vascular land plants. Nature 363 (6 May), 67–69.

    Article  Google Scholar 

  6. Simon, L., Lalonde, M., and Bruns, T. D. (1992) Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl Environ Microbiol 58(1), 291–295.

    PubMed  CAS  Google Scholar 

  7. Simon, L., Lévesque, R. C, and Lalonde, M. (1992) Rapid quantitation by PCR of endomycorrhizal fungi colonizing roots, PCR Methods Appl. 2, 76–80.

    PubMed  CAS  Google Scholar 

  8. Simon, L., Lévesque, R C., and Lalonde, M. (1993) Identification of endomycorrhizal fungi colonizing roots using fluorescent SSCP-PCR. Appl Environ Microbiol 59(12), 4211–4215.

    PubMed  CAS  Google Scholar 

  9. Bousquet, J., Simon, L., and Lalonde, M. (1990) DNA amplification from vegetative and sexual tissues of trees using polymerase chain reaction Can J. Forestry Res 20(2), 254–257.

    Article  CAS  Google Scholar 

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© 1996 Humana Press Inc.

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Simon, L. (1996). Specific PCR Primers for the Identification of Endomycorrhizal Fungi. In: Clapp, J.P. (eds) Species Diagnostics Protocols. Methods in Molecular Biology™, vol 50. Humana Press. https://doi.org/10.1385/0-89603-323-6:187

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  • DOI: https://doi.org/10.1385/0-89603-323-6:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-323-8

  • Online ISBN: 978-1-59259-537-2

  • eBook Packages: Springer Protocols

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