Abstract
The technique of patch-clamp recording in brain slices is applicable to a large variety of cell types in slices from nearly all areas of the central nervous system (CNS) in animals at many different stages of development (Blanton et al., 1989; Edwards et al., 1989; Konnerth, 1990). To date, the technique has been successfully applied in a number of areas of the CNS, including the hippocampus, cerebellum, striatum, brain stem, corpus callosum, hypothalamus, frontal and visual cortex, medial septum, olfactory bulb, retina, and spinal cord. In addition, the technique has been applied to other tissues, including the pituitary gland and cardiac muscle.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Armstrong C. M. and Gilly W. F. (1992) Access resistance and space clamp problems associated with whole-cell patch-clamping. Meth. Enzymol. 207, 100–122.
Barbour B. (1993) Synaptic currents evoked in Purkinje cells by stimulating individual granule cells. Neuron 11, 759–769.
Blanton M. G., Lo T. J., and Kriegstein A. R. (1989) Whole cell recording from neurons in slices of reptilian and mammalian cerebral cortex. J. Neurosci. Meth. 30, 203–210.
Bochet P., Audinat E., Lambolez B., Crepel F. R. J., Iino M., Tsuzuki K., and Ozawa S. (1994) Subunit composition at the single-cell level explains functional properties of a glutamate-gated channel. Neuron 12, 383–388.
Dodt H. U. (1992) Infrared video microscopy of living brain slices, in Practical Electrophysiological Methods (Kettenman H. and Grantyn R., eds.) Wiley-Liss, New York, pp. 6–10
Dodt H. U. and Zieglgansberger W. (1990) Visualizing unstained neurons in living brain slices by infrared DIC-videomicroscopy. Brain Res. 537, 333–336.
Edwards F., Konnerth A., and Sakmann B. (1990) Quanta1 analysis of inhibitory synaptic transmission in the dentate gyrus of rat hippocampal slices: a patch-clamp study. J. Physiol. 430, 213–249.
Edwards F., Konnerth A., Sakmann B., and Takahashi T. (1989) A thin slice preparation for patch-clamp recordings from neurones of the mammalian central nervous system. Pflügers Arch. 414, 600–612.
Eilers J., Schneggenburger R., and Konnerth A. Patch-Clamp and calcium imaging in brain slices, in Single-Channel Recording, 2nd ed. (Sakmann B. and Neher, E., eds.) Plenum, New York, pp. 213–229.
Horn R. and Marty A. (1988) Muscarinic activation of ionic currents measured by a new whole-cell recording method. J, Gen. Physiol. 92, 145–159.
Ito M. (1989) Long-term depression. Ann. Rev. Neurosci. 12, 85–102.
Kano M. and Konnerth A. (1992) Potentiation of GABA-mediated currents by CAMP-dependent protein kinase. Neuro Report 3, 563–566.
Kano M., Rexhausen U., Dreessen J., and Konnerth A. (1992) Synaptic excitation produces a long-lasting rebound potentiation of inhibitory synaptic signals in cerebellar Purkinje cells. Nature 356, 601–604.
Konnerth A. (1990) Patch-clamping neurons in slices of mammalian CNS. Trends Neurosci. 13, 321–323.
Konnerth A., Dreessen J., and Augustine G. J. (1992) Brief dendritlc calcium signals initiate long-lasting synaptic depression. Proc. Natl. Acad. Sci. USA 89, 7051–7055.
Konnerth A., Obaid A. L., and Salzberg B. M. (1987) Optical recording of electrical activity from parallel fibres and other cell types in skate cerebellar slices ”in vitro.” J. Physiol. 393, 681–702.
Lambolez B., Audinat E., Bochet P., Crepél F., and Rossier J. (1992) AMPA receptor subunits expressed by single Purkinje cells. Neuron 9, 247–258.
Levis R. A. and Rae J. L. (1992) Constructing a patch-clamp setup. Meth. Enzymol. 207, 14–66.
Llano I., Marty A., Armstrong C. M., and Konnerth A. (1991) Synapticand agonist-induced excitatory currents of Purkinje cells in rat cerebellar slices. J. Physiol. 434, 183–213.
MacVicar B. A. (1984) Infrared video microscopy to visualize neurons in the in vitro brain slice preparation. J. Neurosci. Meth. 12, 133–139.
Neher E. (1992) Correction for liquid junction potentials in patch-clamp experiments. Meth. Enzymol. 207, 123–131.
Neher E. and Augustine G. J. (1992) Calcium gradients and buffers in bovine chromaffin cells. J. Physiol. 450, 273–301.
Regehr W., Konnerth A., and Armstrong C. M. (1992) Sodium action-potentials in the dendrites of cerebellar Purkinje cells. Proc. Natl. Acad. Sci. USA 89, 5492–5496.
Schneggenburger R., Zhou Z., Konnerth A., and Neher E. (1993) Fractional contribution of calcium to the cation current through glutamate receptor channels. Neuron 11, 133–143.
Spruston N., Jaffe D. B., and Johnston D. (1994) Dendritic attenuation of synaptic potentials: the role of passive membrane properties. Trends. Neurosci. 17, 161–166.
Spruston N. and Johnston D. (1992) Perforated patch-clamp analysis of the passive membrane properties of three classes of hippocampal neurons. J. Neurophysiol. 67, 508–529.
Stuart G. J., Dodt H. U., and Sakmann B. (1993) Patch-Clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflügers Arch. 423, 511–518.
Stuart G. J. and Sakmann B. (1994) Active propagation of somatic action potentials into neocortical pyramidal cell dendrites. Nature 367, 69–72.
Usowicz M. M., Sugimori M., Cherksey B., and Llinás R. (1992) P-type calcium channels in the somata and dendrites of adult cerebellar Purkinje cells. Neuron 9, 1185–1199.
Vincent P. and Marty A. (1993) Neighboring cerebellar Purkinje cells communicate via retrograde inhibition of common presynaptic interneurons. Neuron 11, 885–893.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 The Humana Press Inc.
About this protocol
Cite this protocol
Plant, T.D., Eilers, J., Konnerth, A. (1995). Patch-Clamp Technique in Brain Slices. In: Boulton, A.A., Baker, G.B., Walz, W. (eds) Patch-Clamp Applications and Protocols. Neuromethods, vol 26. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-311-2:233
Download citation
DOI: https://doi.org/10.1385/0-89603-311-2:233
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-311-5
Online ISBN: 978-1-59259-631-7
eBook Packages: Springer Protocols