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Patch-Clamp Recording and RT-PCR on Single Cells

  • Bertrand Lambolez
  • Etienne Audinat
  • Pascal Bochet
  • Jean Rossier
Protocol
Part of the Neuromethods book series (NM, volume 26)

Abstract

The technique described in this chapter provides electrophysiologists using patch-clamp with a convenient method to link electrophysiological data to a molecular analysis of the mRNAs expressed in a single cell. This molecular analysis can be used either to correlate cell responses with their molecular basis or to identify cell types according to the expression of specific markers. The core of the molecular analysis is polymerase chain reaction (PCR), which makes it fast, sensitive, and simple.

Keywords

Polymerase Chain Reaction Purkinje Cell Glial Fibrillary Acidic Protein Polymerase Chain Reaction Amplification Polymerase Chain Reaction Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc. 1995

Authors and Affiliations

  • Bertrand Lambolez
    • 1
  • Etienne Audinat
    • 2
  • Pascal Bochet
    • 3
  • Jean Rossier
    • 3
  1. 1.Znstitut Atfred FessardCentre National de la Recherche ScienttfiqueGif-sur-YvetteFrance
  2. 2.Laborutoire de Neurobiofogie et de Neuropharmacologie du DeveloppementCentre National de la Recherche ScientifiqueOrsayFrance
  3. 3.Institut Alfred FessardCentre National de la Recherche ScientifqueGifsur-YvetteFrance

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