Abstract
The production of transgenic laboratory animals has rapidly gained importance as an experimental tool to study the factors that determine the tissue specificity of gene expression, to generate models for human diseases (1,2), as well as to look at the consequences of oncogene expression (3,4). Through this technique, recombinant DNA molecules of any type can be introduced into zygotes for use in producing transgenic domestic animals. Introduction of foreign genes into early embryos, particularly into bovine embryos, may be a powerful tool to obtain more valuable animals. Although pronuclear microinjection is the only proven route for gene transfer in livestock, the production of transgenic animals is still problematic and inefficient (5), and importantly, microinjection may impair the development of the embryos (6).
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References
Léonard, J. M., Abramczuk, J. W., Pezen, D. S., Rutledge, R., Belcher, J. H., Hakim, F., Shearer, G., Lamperth, L., Travis, W., Fredrickson, T., Motkins, A. L., and Martin, M. A. (1988) Development of disease and virus recovery in transgenic mice containing HIV proviral DNA. Science 242, 1665–1670.
Small, J. A., Scangos, G. A., Cork, L., Jay, G., and Khoury, G. (1986) The early region of hyman papovavirus JC induces dysmyelination in transgenic mice. Cell 46, 13–18.
Andres, A. C., Schonenberger, C. A., Groner, B., Hennighousen, L., Lemeur, M., and Gerlinger, P. (1987) Ha-ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation and tumor induction in transgenic mice. Proc. Natl. Acad. Sci. USA 84, 1299–1303.
Sinn, E., Muller, W., Pattengale, P., Tepler, I., Wallace, R., and Leder, P. (1987) Coexpression of MMTV/V Ha-Ras and MMTV/c-myc genes in transgenic mice: synergistic action of oncogenes in vivo. Cell 49, 465–475.
Clark, A. J., Simons, J. P., and Wilmut, I. (1992) Germ line manipulation: applications in agriculture and biotechnology, in Transgenic Animals (Grosveld, F., and Kollias, G., eds.), Academic, London, pp. 249–270.
Gagné, M., Pothier, F., and Sirard, M. A. (1990) Developmental potential of early bovine zygotes submitted to centrifugation and microinjection following in vitro maturation of oocytes. Theriogenology 34, 417–425.
Knutson, J. C., and Yee, D. (1987) Electroporation: parameters affecting transfer of DNA into mammalian cells. Anal. Biochem. 164, 44–52.
Neumann, E., Schaefer-Rider, M., Wang, Y., and Hofschneider, P. H. (1982) Gene transfer into mouse lyoma cells by electroporation in high electric field. EMBO J. 7, 841–845.
Evans, G. A., Ingraham, H. A., Lewis, K., Cunningham, K., Seki, T., Moruichi, T., Chang, H. C., Silver, L., and Hyman, R. (1984) Expression of the thy-1-glycoprotein gene by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 81, 5532–5536.
Miller, J. F., Dower, W. J., and Thompkins, L. S. (1988) High-voltage electroporation of bacteria: genetic transformation of campylobacter jejuni with plasmid DNA. Proc. Natl. Acad. Sci. 85, 856–860.
Lavitrano, M., Canaioni, A., Fazio, V. M., Dolci, S., Farace, M. G., and Spadafora, C. (1989) Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice. Cell 57, 717–723.
Parrish, J. J., Susko-Parrish, J. L., and First, N. L. (1988) Capacitation of bovine sperm by heparin. Biol. Reprod. 38, 1171–1180.
Zimmerman, U. and Vienken, J. (1982) Electric field-induced cell-to-cell fusion. Membr. Biol. 67, 165–182.
Gagné, M., Pothier, F., and Sirard, M. A. (1991) Electroporation of bovine spermatozoa to carry foreign DNA in oocytes. Mol. Reprod. Dev. 29, 6–15.
Sirard, M. A., Leibfried-Rutledge, L., Parrish, J., Ware, C., and First, N. L. (1988) The culture of bovine oocytes to obtain developmentally competent embryos. Biol. Reprod. 39, 546–552.
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© 1995 Humana Press Inc.
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Gagné, M., Pothier, F., Sirard, MA. (1995). The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes. In: Nickoloff, J.A. (eds) Animal Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology, vol 48. Humana Press. https://doi.org/10.1385/0-89603-304-X:161
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DOI: https://doi.org/10.1385/0-89603-304-X:161
Publisher Name: Humana Press
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Online ISBN: 978-1-59259-535-8
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