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Agrobacterium-Mediated Transformation of Potato Genotypes

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 44)

Abstract

The potato (Solanum tuberosum) is the world’s fourth major food crop. The potato is ideally suited for improvement by modern genetic manipulation methods, as it is highly amenable to a wide range of various tissue-culture techniques (1). In recent years, a combination of cellular and molecular approaches, including somatic hybridization and genetic transformation, have been employed to improve existing potato cultivars (2). Genetic transformation, however, provides a more direct and controlled method for manipulating the genome of potato for both basic and applied research purposes (3). This chapter describes the Agrobacterium-mediated transformation method for potato genotypes. Agrobacterium tumefaciens and its Ti-plasmid have been used extensively as vectors to introduce engineered genes into plants (4). In order for genetic modification via Agrobacterium to be successful, both a reproducible regeneration system and an efficient DNA delivery system directed toward those cells capable of regeneration are mandatory. An ability to select the transformed cells from a large number of untransformed cells is also essential, because transformation frequency is usually between 5 and 50%.

Keywords

MS30 Medium Somatic Hybridization MS30 Liquid Medium Zeatin Riboside Potato Genotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc., Totowa, NJ 1995

Authors and Affiliations

  1. 1.Department of Soft Fruit Genetics and Cell and Molecular GeneticsScottish Crop Research InstituteInvergowrie, DundeeScotland

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