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Autoradiography of β1-and β2-Adrenoceptors

  • Roger J. Summers
  • Peter Molenaar
Part of the Methods in Molecular Biology™ book series (MIMB, volume 41)

Abstract

Receptor autoradiography is derived from receptor binding techniques that were originally developed for use in homogenate membranes or isolated cells. The use of tissue sections has the advantage of retaining anatomical information that can provide useful insights into cell signaling systems involved in neurotransmitter receptor interactions. Like homogenate membrane or whole-cell binding techniques, receptor autoradiography can also be used to derive kinetic, saturation, competition binding, and receptor density data. This chapter describes methods for receptor autoradiography using specific ligands that can be used to localize, characterize, and quantitate ß1- and ß2-adrenoceptors in anatomically defined regions.

Keywords

Nuclear Emulsion Homogenate Membrane Quantitative Autoradiography Potassium Alum Sodium Iodate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Engel, G., Hoyer, D., Berthold, D., and Wagner, H. (1981) (f)[125Iodo]-cyanopindolol, a new ligand for ß-adrenoceptors: identification and quantitation of subclasses of P-adrenoceptors in guinea-pig. Naunyn-Schmeideberg’s Arch. Pharmacol. 317, 277–285.CrossRefGoogle Scholar
  2. 2.
    Buxton, B. F., Jones, C. R., Molenaar, P., and Summers, R. J. (1987) Characterization and autoradiographic localization of ß-adrenoceptor subtypes in human cardiac tissues. Br. I. Pharmacol. 92, 299–310.Google Scholar
  3. 3.
    Molenaar, P., Canale, E., and Summers, R. J. (1987) Autoradiographic localization of ß1-and ß2-adrenoceptors in guinea-pig atrium and regions of the conducting system. J. Pharmacol. Exp. Ther. 241, 1048–1064.PubMedGoogle Scholar
  4. 4.
    Molenaar, P., Jones, C. R., McMartin, L. R., and Summers, R. J. (1988) Autoradiographic localization and densitometric analysis of ß1-and ß2-adrenoceptors in the canine left anterior descending coronary artery. J. Pharmacol. Exp. Ther. 246, 384–393.PubMedGoogle Scholar
  5. 5.
    Young, W. S. and Kuhar, M. J. (1979) A new method for receptor autoradiography: [3H]-opioid receptors in rat brain. Brain Rex 179, 255–270.CrossRefGoogle Scholar
  6. 6.
    Molenaar, P., Malta, E., Jones, C. R., Buxton, B. F., and Summers, R. J. (1988) Autoradiographic localization and function of ß-adrenoceptors on the human internal mammary artery and saphenous vein. Br. J. Pharmacol. 95, 225–233.PubMedGoogle Scholar
  7. 7.
    Russell, F. D., Molenaar, P., Edyvane, N., Smolich, J. J., and Summers, R. J. (1991) Autoradiographic localization and quantitation of ß-adrenoceptor subtypes in the guinea-pig sinoatrial node. Mol. Neuropharmacol. 1, 141–147.Google Scholar
  8. 8.
    Molenaar, P., Russell, F. D., Shimada, T., and Summers, R. J. (1990) Densitometric analysis of ß1-and ß2-adrenoceptors in guinea-pig atrioventricular conducting system. J. Mol. Cell. Cardiol. 22, 483–495.PubMedCrossRefGoogle Scholar
  9. 9.
    Molenaar, P., Smolich, J. J., Russell, F. D, McMartin, L. R., and Summers, R. J. (1990) Differential regulation of beta-1 and beta-2 adrenoceptors in guinea-pig atrioventricular conducting system after chronic (−)-isoproterenol infusion. J. Pharmacol. Exp. Ther. 255, 393–400PubMedGoogle Scholar
  10. 10.
    Kompa, A., Molenaar, P., and Summers, R. J. (1991) Regulation of cardiac ß-adrenoceptor subtypes in guinea-pig after (−)-adrenaline and (−)-noradrenaline treatment. Mol. Neuropharmacol. 1, 203–210.Google Scholar
  11. 11.
    Elnatan, J., Molenaar, P., Rosenfeldt, F. L., and Summers, R. J. (1994) Autoradiographic localization and quantitation of ß1-and ß2-adrenoceptors m the human atrioventricular conducting system: a comparison of patients with idiopathic dilated cardiomyopathy and ischemic heart disease. J. Mol. Cell. Cardiol. 26, 313–323.PubMedCrossRefGoogle Scholar
  12. 12.
    Miller, J. A., Curella, P., and Zahniser, N. R. (1988) A new densitometric procedure to measure protein levels in tissue slices used in quantitative autoradiography. Brain Res. 447, 60–66.PubMedCrossRefGoogle Scholar
  13. 13.
    Munson, P. J. and Rodbard, D. (1980) LIGAND: a versatile computerized approach for the characterization of ligand binding systems. Anal. Biochem. 107, 220–239.PubMedCrossRefGoogle Scholar
  14. 14.
    Neve, K. A., McGonigle, P., and Molinoff, P. B. (1986) Quantitative analysis of the selectivity of radioligands for subtypes of beta adrenergic receptors. J. Pharmacol. Exp. Ther. 238, 46–53.PubMedGoogle Scholar
  15. 15.
    Murphree, S. S. and Saffitz, J. E. (1988) Delineation of the distribution of p-adrenergic receptor subtypes in canine myocardium. Circ. Res. 63, 117–125.PubMedGoogle Scholar
  16. 16.
    Williams, D. W. and Summers, R. J. (1990) SIMUL: an accurate method for the determination of receptor subtype proportions using a personal computer. Comp. Prog. Biomed. 32, 137–139.CrossRefGoogle Scholar
  17. 17.
    Molenaar, P., Kompa, A., Roberts, S. J., Pak, H. S., and Summers, R. J. (1992) Localization of (−)-[125I]-cyanopindolol binding in guinea-pig heart: characteristics of non-ß-adrenoceptor related binding in cardiac pacemaker and conducting regions. Neurosci. Lett. 136, 118–122.PubMedCrossRefGoogle Scholar
  18. 18.
    Molenaar, P., Kim, Y. S., Roberts, S. J., Pak, H., Sainz, R. D., and Summers, R. J. (1991) Localization and characterization of two propranolol resistant (−)[125I]cyanopindolol binding sites in rat skeletal muscle. Eur. J. Pharmacol. 209, 257–262.PubMedCrossRefGoogle Scholar
  19. 19.
    Roberts, S. J., Molenaar, P., and Summers, R. J. (1993) Characterisation of propranolol-resistant (−)-(125I]-cyanopindolol binding sites in rat soleus muscle. Br. J. Pharmacol. 109, 344–352.PubMedGoogle Scholar
  20. 20.
    Russell, F. D., Molenaar, P., and Summers, R. J. (1992) Absence of mitochondrial P-adrenoceptors in guinea-pig myocardium: evidence for tissue disparity. Gen. Pharmacol. 23, 827–832.PubMedGoogle Scholar

Copyright information

© Humana Press Inc , Totowa, NJ 1995

Authors and Affiliations

  • Roger J. Summers
    • 1
  • Peter Molenaar
    • 1
  1. 1.Department of PharmacologyUniversity of MelbourneParkvilleAustralia

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