Mass Measurement of Key Phosphoinositide Cycle Intermediates

  • R. A John Challiss
Part of the Methods in Molecular Biology™ book series (MIMB, volume 41)


The phosphoinositide cycle is now established as a major pathway by which a variety of hormones, neurotransmitters, and other signaling molecules can generate second messengers and thus affect the intracellular milieu (1). Receptor-mediated activation of membrane-associated enzymic activities act on the minor membrane phospholipid phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] to generate three second messenger molecules: inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and 1,2-diacylglycerol via inositol phospholipid-specific phospholipase C (PI-PLC) activity, and phosphatidylinositol 3,4,5-trisphosphate via phosphoinositide 3-kinase activity (2).


Wash Buffer Inositol Phosphate Mass Determination Inositol Phospholipid Radioisotopic Label 
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Copyright information

© Humana Press Inc , Totowa, NJ 1995

Authors and Affiliations

  • R. A John Challiss
    • 1
  1. 1.Department of Cell Physiology and PharmacologyUniversity of LeicesterUK

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