Skip to main content

Freeze-Drying and Cryopreservation of Bacteria

  • Protocol
Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 38))

Abstract

Freezing and freeze-drying techniques have become standard methods for the long-term maintenance of bacterial cultures. Both methods of preservation provide varying degrees of success with different species of bacteria, and neither technique results in 100% recovery of preserved cells (1). There is no universally applicable method for the successful preservation of all bacteria, and where it is vitally important that cultures are not lost, it is advisable to use both methods in parallel.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Kirsop, B. (1985) The current status of culture collections and theu contribution to biotechnology. Crit. Rev. Biotechnol. 2, 287–314.

    Article  Google Scholar 

  2. Franks, F. (1990) Freeze-drying: from empiricism to predictability. Cryo-Lett. 11, 93–110.

    Google Scholar 

  3. Meryman, H T. (1966) Freeze-drying, in Cryobiology (Meryman, H. T., ed.), Academic, London, pp. 609–663.

    Google Scholar 

  4. Mackey, B. M. (1984) Lethal and sublethal effects of refrigeration, freezing and freeze-drying on micro-organisms, in The Revtval of Iqured Microbes (Andrew, M. E. E. and Russell, A. D., eds.), Academic, London, Society of Applied Bacteriology Symposium series, No. 12.

    Google Scholar 

  5. Crowe, J. H., Carpenter, J. F., Crowe, L. M., and Anchordoguy, T. J. (1990) Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiology 27, 219–231.

    Article  CAS  Google Scholar 

  6. Kirsop, B. E. and Doyle, A. (eds.) (1991) Maintenance of Micro-Organisms and Cultured Cells: A Manual of Laboratory Methods, 2d ed., Academic, London.

    Google Scholar 

  7. Rudge, R. H. (1991) Maintenance of bacteria by freeze-drying, in Maintenance of Macro-Organisms and Cultured Cells: A Manual of Laboratory Methods, 2d ed. (Kirsop, B. E. and Doyle, A., eds.), Academic, London, pp. 31–44.

    Google Scholar 

  8. Heckly, R. J. (1978) Preservation of micro-organisms. Adv. Appl. Microb. 3, 1–76.

    Article  Google Scholar 

  9. Morris, G. J, Coulson, G. E., and Clarke, K. J. (1988) Freezing injury in Saccharomyces cerevisiae: The effect of growth conditions. Cryobiology 25, 471–482.

    Article  Google Scholar 

  10. Grout, B. W. W. and Morris, G. J. (1987) Freezing and cellular organization, in The Effects of Low Temperatures on Biological Systems (Grout, B. W. W. and Morris, G. J., eds.), Edward Arnold, London, pp. 147–174.

    Google Scholar 

  11. Feltham, R. K. A., Power, A. K., Pell, P. A., and Sneath, P. H. A. (1978) A simple method for storage of bacteria at −76°C. J. Appl. Bact. 44, 313–316.

    Article  CAS  Google Scholar 

  12. Redway, K. F. and Lapage, S. P. (1974) Effect of carbohydrates and related compounds on the long-term preservation of freeze-dried bacteria. Cryobiology 11, 73–79

    Article  CAS  PubMed  Google Scholar 

  13. Barnes, E. M. (1969) Methods for the gram-negative non-sporing anaerobes, in Methods in Microbiology, vol 3B (Norris, J. R. and Ribbons, D. W., eds.), Academic, New York, pp. 151–160.

    Google Scholar 

  14. Malik, K. A. (1990) Use of activated charcoal for the preservation of anaerobic phototropic and other sensitive bacteria by freeze-drying. J Microbial. Methods 12, 117–124.

    Article  Google Scholar 

  15. Nei, T., Souzu, H., and Araki, T. (1966) Effect of residual moisture content on the survival of freeze-dried bacteria during storage under various conditions. Cryobiology 2, 276–279.

    Article  CAS  PubMed  Google Scholar 

  16. Mazur, P. (1977) The role of intracellular freezing in the death of cells cooled at supraoptimal rates. Cryobiology 14, 251–272.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Humana Press Inc.

About this protocol

Cite this protocol

F., S. (1995). Freeze-Drying and Cryopreservation of Bacteria. In: Day, J.G., Pennington, M.W. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology™, vol 38. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-296-5:21

Download citation

  • DOI: https://doi.org/10.1385/0-89603-296-5:21

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-296-5

  • Online ISBN: 978-1-59259-525-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics