Abstract
Since the first reports of the Philadephia chromosome in chronic myelogenous leukemia (CML), nonrandomly occurring chromosome abnormalities have been described in a number of leukemias and lymphomas (1,2). The identification of the DNA sequences that span the translocation breakpoints in several hematological malignancies, and the cloning and sequencing of the inmiunoglobulin and T-cell receptor genes, have created the ability to reliably diagnose and monitor these diseases with molecular-based techniques that complement the analysis of banded metaphase chromosomes (3).
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References
Nowell, P. C and Hungerford, D A. (1960) A minute chromosome in human granulocytic leukemia. Science 132, 1497.
Heim, S. and Mitelman, F. (1987) Cancer Cytogenetics. Liss, New York
Davey, M. P., Bongiovanni, K. F., Kaulfersh, W., Quertemous, T., Seidman, J. H, Hersfield, M. S., Kurtzberg, J, Haynes, B, Davis, M M., and Waldmann, T. A. (1986) Immunoglobulin and T-cell receptor gene rearrangement and expression m human lymphoid leukemia cells at different stages of maturation. Proc. Natl Acad. Sci. USA 83, 8759–8763.
Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis J. Mol Biol 98, 503–517.
Blin, N and Stafford, D W. (1976) Isolation of high molecular weight DNA. Nucleic Acids Res. 3, 2303–2308
Johnson, P H, Miller, M J., and Grossman, L. I (1980) Electrophoresis of DNA in agarose gels II. Effects of loading mass electroendosmosis on elec-trophoretic mobilities. Anal Biochem 102, 159–162
Wahl, G. M, Stern, M, and Stark, G R. (1979) Efficient transfer of large DNA fragments from agarose gels to DBM paper and rapid hybndization using dextran sulfate Proc. Natl Acad. Sci. USA 76, 3683–3687
Medveczky, P., Chang, P. C, Oste, C, and Mulder, C (1987) Rapid vacuum driven transfer of DNA and RNA from gels to solid supports BioTechniques 5, 242–246
Church, G and Gilbert, W (1984) Genomic sequencing Proc Natl. Acad Sci USA 81, 1991–1995.
Feinberg, A. P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity Anal. Biochem. 136, 6–13
Leary, J. J, Brigati, D. J, and Ward, D C. (1983) Rapid and sensitive colormetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots Proc Natl. Acad. Sci USA 80, 4045–4049.
Sealy, P. G, Whittaker, P. A, and Southern, E M. (1985) Removal of repeated sequences from hybridization probes Nucleic Acids Res. 13, 1905–1922.
Church, G and Gilbert, W (1984) Genomic sequencing Proc Natl Acad. Sci. USA 81, 1991–1995
Casey, J. and Davidson, N (1977) Rates of formation and thermal stabilities of RNA’DNA and DNA’DNA duplexes at high concentrations of formamide Nucleic Acids Res. 4, 1539–1552.
Sambrook, J., Fritsch, E. F, and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
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© 1994 Humana Press Inc, Totowa, NJ
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Saltman, D.L., Hunger, S.P., Turner, G.E. (1994). Molecular Analysis of Chromosome Aberrations in Hematological Malignancies. In: Gosden, J.R. (eds) Chromosome Analysis Protocols. Methods in Molecular Biology™, vol 29. Humana Press. https://doi.org/10.1385/0-89603-289-2:437
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DOI: https://doi.org/10.1385/0-89603-289-2:437
Publisher Name: Humana Press
Print ISBN: 978-0-89603-289-7
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