Abstract
This chapter gives general information on common practical features of the ELISA, featuring the main elements of:
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1.
The adsorption of antigen or antibody to the plastic solid-phase.
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2.
The addition of the test sample and subsequent reagents.
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3.
The incubation of reactants.
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4.
The separation of bound and free reactants by washing.
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5.
The addition of enzyme-labeled reagent.
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6.
The addition of enzyme detection system (color development).
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7.
The visual or spectrophotometric reading of the assay.
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© 1995 Humana Press Inc.
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Crowther, J.R. (1995). Stages in ELISA. In: Crowther, J.R. (eds) ELISA. Methods in Molecular Biology™, vol 42. Humana Press. https://doi.org/10.1385/0-89603-279-5:63
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DOI: https://doi.org/10.1385/0-89603-279-5:63
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