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Procedures to Improve Difficult Couplings

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Book cover Peptide Synthesis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 35))

Abstract

The successful coupling of amino acid derivatives during the synthesis of a peptide by either solution or solid-phase procedures depends on both the reactivity of the carboxyl group of the N-protected amino acid and the steric accessibility of the reactive nucleophile (either a primary or secondary amine). Activation of the carboxyl group is a requisite for the synthesis of an amide bond. Many activation procedures have been developed to accomplish this, and ultimately, the reactivity of the activated species is crucial in determining the coupling yield.

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Author information

Authors and Affiliations

  1. Bachem Bioscience, King of Prussia, PA, USA

    Michael W. Pennington & Michael E. Byrnes

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  1. Michael W. Pennington
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  2. Michael E. Byrnes
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Editors and Affiliations

  1. Bachem Bioscience, King of Prussia, PA, USA

    Michael W. Pennington

  2. University of Florida, Gainesville, FL, USA

    Ben M. Dunn

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© 1994 Humana Press Inc.

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Pennington, M.W., Byrnes, M.E. (1994). Procedures to Improve Difficult Couplings. In: Pennington, M.W., Dunn, B.M. (eds) Peptide Synthesis Protocols. Methods in Molecular Biology, vol 35. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-273-6:1

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  • DOI: https://doi.org/10.1385/0-89603-273-6:1

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-273-6

  • Online ISBN: 978-1-59259-522-8

  • eBook Packages: Springer Protocols

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