DNA-Protein Interactions pp 211-225 | Cite as
Site-Directed and Site-Saturation Mutagenesis Using Oligonucleotide Primers
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Abstract
A very powerful method of probing DNA-protein interactions is the technique of site-directed mutagenesis. Using this approach one can introduce specific amino acid changes at any given position in the amino acid sequence of a DNA-binding protein, and test the functional consequences of these mutations in vitro. Furthermore, it is also possible to investigate the resulting mutations in vivo if a suitable phenotypic change can be measured. If so, strategies that introduce random base changes to a given codon (or indeed to a larger region of the protein) are particularly valuable.
Keywords
Single Base Substitution Sodium Acetate Solution Ethidium Bromide Solution Primer Extension Reaction Mutagenic Oligonucleotide
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information
© Humana Press Inc. 1994