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Models to Evaluate Effects of Neurotrophic Factors on Axonal Regeneration

  • Protocol
Neurotrophic Factors

Part of the book series: Neuromethods ((NM,volume 25))

Abstract

An injury to neural tissue that disrupts axonal continuity causes disconnection of pre- and postsynaptic elements of a neuronal circuit. The axon also transports to the cell body neurotrophic factors from the innervation territory, and axotomy, by interrupting this trophic supply, may lead to degeneration or death of the neuron unless supplementary trophic factors are available. Neural regeneration could be broadly defined as a process leading to the restitution of function in the damaged circuit. Such a process would involve, among other aspects, the maintenance of afferents to the presynaptic element, the regrowth of an axon, selection of and connection to the correct postsynaptic partner, and the promotion or restoration of downstream circuits to which this postsynaptic element contributes. Axonal regeneration, the topic of this chapter, is only concerned with the new growth of an axon in the direction of a putative postsynaptic partner.

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References

  • Aebischer P. (1989) The role of biomaterlals in peripheral nerve regeneration, in Tissue Engineering (R. Skalok and C.F. Fox, eds.) Alan Liss, New York,pp 257–262.

    Google Scholar 

  • Aebischer P., Salesslotis A.N., and Winn S. R. (1989) Basic fibroblast growth factor released from synthetic guidance channels facilitates peripheral nerve regeneration across long nerve gaps J. Neurosci Res. 23, 282–289

    Article  PubMed  CAS  Google Scholar 

  • Aguayo A.J., Dickson R., Trecarten J., Attiwell M., Bray G.M., and Richardson P. (1978) Ensheathment and myelination of regenerating PNS fibers by transplanted optic nerve glia. Neuroscl. Lett. 9, 97–104.

    Article  CAS  Google Scholar 

  • Aguayo A.J. (1985) Capacity for renewed axonal growth in the mammalian central nervous system, in Central Nervous System Plasticity and Repair (Bignami A., Bloom F. E., Bolis C. L., and Adeloye A., eds.) Raven Press, New York, pp. 31–40.

    Google Scholar 

  • Aguayo A. J., Rasminsky M., Bray G. M., Carbonetto S., McKerracher L., Villegas P. M., Vidal S.M., and Carter D. A. (1991) Degenerative and regenerative responses of injured neurons in the central nervous system of adult mammals. Philos. Trans. R. Sot. Lund. [Biol.] 331, 337–343.

    Article  CAS  Google Scholar 

  • Archibald S. J., Krarup C., Shefner J., Li S.T., and Madison R. D. (1991) A collagen-based nerve guide conduit for peripheral nerve repair: an electrophysiological study of nerve regeneration in rodents and nonhuman primates. J. Camp. Neural. 306, 685–696.

    Article  CAS  Google Scholar 

  • Bates D. J., Ranford J. A., and Mangelsdorf D. C. (1991) Blot and culture analysis of neuronotrophic factors in nerve regeneration chamber fluids. Neurochem. Res. 16, 621–628.

    Article  PubMed  CAS  Google Scholar 

  • Bothwell M. (1991) Keeping track of neurotrophin receptors. Cell 65, 915–918.

    Article  PubMed  CAS  Google Scholar 

  • Campenot R. B. (1982) Development of sympathetic neurons in compartmentalized cultures. I. Local control of neurite growth by nerve growth factor. Dev. Biol. 93, 1–12.

    Article  PubMed  CAS  Google Scholar 

  • Carmignoto G., Maffei L., Candeo P., Canella R., and Comelli C. (1989) Effect of NGF on the survival of rat retinal ganglion cells following optic nerve section. J. Neurosci. 9, 1263–1272.

    PubMed  CAS  Google Scholar 

  • Carnow T. B., Manthorpe M., Davis G.E., and Varon S. (1985) Localized survival of ciliary ganglionic neurons identifies neuronotrophic factor bands on nitrocellulose blots, J. Neuroscr. 5, 1965–1971.

    CAS  Google Scholar 

  • Cordelro P. G., Seckel B. R., Lipton S. A., D’Amore P. A., Wagner J., and Madison R. (1989) Acidic fibroblast growth factor enhances peripheral nerve regeneration in vivo. Pkst. Reconstr. Surg. 83, 1013–1019; discussion 1020-1021.

    Article  Google Scholar 

  • Danielsen N., Muller H., Pettmann B., Williams L. R., Davis G. E., Engvall E., Manthorpe M., and Varon S. (1988a) Rat amnion membrane matrix as a substratum for regenerating axons from peripheral and central neurons: effects in a silicone chamber model. Dev. Bruin Res. 39, 39–50.

    Article  Google Scholar 

  • Danielsen N., Pettmann B., Vahlsing H. L., Manthorpe M., and Varon S. (1988b) Fibroblast growth factor effects on peripheral nerve regeneration in a silicone chamber model. J. Neurosci. Res. 20, 320–330.

    Article  PubMed  CAS  Google Scholar 

  • DaSilva C.F. and Langone F. (1989) Addition of nerve growth factor to the interior of a tubular prosthesis increases sensory neuron regeneration in vivo. Braz.J. Med. Biol. Res. 22, 691–694.

    CAS  Google Scholar 

  • DaSilva C. F., Lima G.M., and Trezena A. G. (1990) Local administration of interleukin-1 increases sensory neuron regeneration in vivo. Bruz. J, Med. Biol. Res. 23, 981–984.

    CAS  Google Scholar 

  • Davis G.E., Varon S., Engvall E., and Manthorpe M. (1985). Substratumbinding neurite-promoting factors: relationships to laminin. Trends Neurosci. 8: 528–532.

    Article  CAS  Google Scholar 

  • Davis G. E., Engvall E., Varon S., and Manthorpe M. (1987a) Human amnion membrane as a substratum for cultured peripheral and central nervous system neurons. Dat. Brurn Res. 33, 1–10.

    Article  Google Scholar 

  • Davis G. E., Blaker S. N., Engvall E., Varon S., Manthorpe M., and Gage F. H. (1987b) Human amnion membrane serves as a substratum for growing axons in vitro and in viva. Science 236, 1106–1109.

    Article  PubMed  CAS  Google Scholar 

  • Dunnett S. B., Rogers D.C., and Richards S. J. (1989) Nigrostriatal reconstruction after 6-OHDA lesions in rats: combination of dopamine-rich nigral grafts and nigrostriatal “bridge” grafts. Exp. Bruin Res. 75, 523–535.

    CAS  Google Scholar 

  • Engvall E., Earwicker D., Manthorpe M., Varon S., and Hagg T. (1991) Merosin, but not lammin is expressed in the mammalian CNS. Soc. Neurosci Abstr. 17, 208.

    Google Scholar 

  • Ernfors P., Ebendal T., Olson L, Mouton P., Stromberg I., and Persson H. (1989a) A cell line producmg recombinant nerve growth factor evokes growth responses in intrimsic and grafted central cholinergic neurons Proc. Natl. Acad. Sn. USA 86, 4756–4760.

    Article  CAS  Google Scholar 

  • Ernfors P., Henschen A., Olson L., and Persson H. (1989b) Expression of nerve growth factor receptor mRNA is developmentally regulated and increased after axotomy in rat spinal cord motoneurons. Neuron 2, 1605–1613.

    Article  PubMed  CAS  Google Scholar 

  • Fernandez E., Pallmi R., and Mercanti D. (1990) Effects of topically administered nerve growth factor on axonal regeneration in peripheral nerve autografts implanted in the spinal cord of rats. Neurosurgery 26, 37–42.

    Article  PubMed  CAS  Google Scholar 

  • Fields R. D., LeBeaci J. M., Longo F. M., and Ellisman M. H. (1989) Nerve regeneration through artificial tubular implants. Prog. Neurobiol, 33, 87–134.

    Article  PubMed  CAS  Google Scholar 

  • Gage F. H., Blaker S. N., Davis G. E., Engvall E., Varon S., and Manthorpe M. (1988) Human amnion membrane matrix as a substratum for axonal regeneration in the central nervous system. Exp. Brain Res, 72, 371–380.

    Article  PubMed  CAS  Google Scholar 

  • Guenard V., Dinarello C. A., Weston P.J., and Aebischer P. (1991) Peripheral nerve regeneration is impeded by interleukin-1 receptor antagonist released from a polymeric guidance channel. J. Neurosci. Res. 29, 396–400.

    Article  PubMed  CAS  Google Scholar 

  • Gundersen R.W. and Barrett J. N. (1980) Characterization of the turning response of dorsal-root neurites toward nerve growth factor. J. Cell Biol. 87, 546–554.

    Article  PubMed  CAS  Google Scholar 

  • Hagg T., Fass H. B., Vahlsing H. L., Manthorpe M., Conner J.M., and Varon S. (1989a) Nerve growth factor (NGF) reverses axotomy-mduced decreases in choline acetyltransferase, NGF receptor and size of medial septum cholmergic neurons. Bram Res. 505, 29–38.

    Article  CAS  Google Scholar 

  • Hagg T., Muir D., Engvall E., Varon S., and Manthorpe M. (1989b) Lamininlike antigen in rat CNS neurons: distribution and changes upon brain injury and nerve growth factor treatment. Neuron 3, 721–732.

    Article  PubMed  CAS  Google Scholar 

  • Hagg T., Vahlsing H. L., Manthorpe M., and Varon S. (1990a) Septohippocampal cholinergic axonal regeneration through peripheral nerve bridges: quantification and temporal development. Exp. Neural. 109, 153–163.

    Article  CAS  Google Scholar 

  • Hagg T., Vahlsing H. L, Manthorpe M., and Varon S. (1990b) Nerve growth factor infusion into the denervated adult rat hippocampal formation promotes its cholinergic reinnervation. J. Neurosci. 10, 3087–3092.

    PubMed  CAS  Google Scholar 

  • Hagg T., Gulati A. K., Behzadian M. A., Vahlsing H. L., Varon S., and Manthorpe M. (1991) Nerve growth factor promotes CNS axonal regeneration into acellular peripheral nerve grafts. Exp. Neural. 112, 79–88.

    Article  CAS  Google Scholar 

  • Hagg T., Quon D., Higalu J., and Varon S. (1992a) Ciliary neuronotrophic factor prevents neuronal degeneration and promotes low-affinity NGF receptor expression in the adult rat CNS. Neuron 8, 145–158.

    Article  PubMed  CAS  Google Scholar 

  • Hagg T, Louis J.C., and Varon S. (1992b) Neurotrophic factors and CNS regeneration, in Neuroregenerution (Gorio A., ed.) Raven Press, New York, pp. 265–287.

    Google Scholar 

  • Hagg T., Rende M., Magal E., Burnham P., Oudega M., and Varon S. (1992c) Potential regulation by trophic factors of low-affinity NGF receptors in spinal motor neurons. Brain Res. Bull. (in press).

    Google Scholar 

  • Hammarback J. A., McCarthy J, B., Palm S. L., Furcht L.T., and Letourneau P.C. (1988) Growth cone guidance by substrate-bound laminin pathways is correlated with neuron-to-pathway adhesivity. Dev. Blol. 126, 29–39.

    Article  CAS  Google Scholar 

  • Hefti F. (1986) Nerve growth factor promotes survival of septal cholinergic neurons after fimbrial transections. J. Neurosci. 6, 2155–2162.

    PubMed  CAS  Google Scholar 

  • Houle J.D. and Johnson J. E. (1989) Nerve growth factor (NGF)-treated nitrocellulose enhances and directs the regeneration of adult rat dorsalroot axons through intraspinal neural tissue transplants. Neurosci. Lett. 103, 17–23.

    Article  PubMed  CAS  Google Scholar 

  • Jenq C.-B. and Coggeshall R. E. (1987) Permeable tubes increase the length of the gap that regenerating axons can span. Brain Res. 409, 239–242.

    Article  Google Scholar 

  • Koliatsos V. E., Crawford T.O., and Price D. L. (1991) Axotomy induces nerve growth factor receptor immunoreactivity in spinal motor neurons. Brain Res. 549, 297–304.

    Article  PubMed  CAS  Google Scholar 

  • Kromer L. F., Bjorklund A., and Stenevi U. (1981) Regeneration of the septohippocampal pathways in adult rats is promoted by utilizing embryonic hippocampal implants as bridges. Bruin Res. 210, 173–200.

    Article  CAS  Google Scholar 

  • Kromer L.F. and Cornbrooks C. J. (1987) Identification of trophic factors and transplanted cellular environments that promote CNS axonal regeneration. Ann. NY Acad. Sci. 495, 207–224.

    Article  PubMed  CAS  Google Scholar 

  • Kunkel-Bagden E. and Bregman B. S. (1990) NGF prevents the retrograde cell loss of red nucleus neurons after spinal cord injury at birth. Soc. Neurosci. Abstr. 16, 479.

    Google Scholar 

  • Liesi P. and Silver J. (1988) Is astrocyte laminin involved in axon guidance in the mammalian CNS? Deu. Biol. 130, 774–785.

    Article  CAS  Google Scholar 

  • Lindholm D., Heumann R., Meyer M., and Thoenen H. (1987) Interleukin-1 regulates synthesis of nerve growth factor in nonneuronal cells of rat sciatic nerve. Nature 330, 658–659.

    Article  PubMed  CAS  Google Scholar 

  • Longo F. M., Skaper S. D., Manthorpe M., Williams L. R., Lundborg G., and Varon S. (1983) Temporal changes m neuronotrophic activities accumulating in vivo within nerve regeneration chambers. Exp. Neural. 81, 756–769.

    Article  CAS  Google Scholar 

  • Longo F. M., Hayman E. G., Davis G. E., Ruoslahti E., Engvall E., Manthorpe M., and Varon S (1984a) Neurite-promoting factors and extracellular matrix components accumulating in vivo within nerve regeneration chambers. Bratn Res. 309, 105–117.

    Article  CAS  Google Scholar 

  • Longo F.M., Selak I., Zovickian J., Manthorpe M, Varon S., and U, H-S (1984b). Neuronotropluc activies in cerebrospinal fluid of head trauma pahents. Exp. Neural 84, 207–218.

    Article  CAS  Google Scholar 

  • Lundborg G, Dahlin L.B., Danielsen N., Hansson H.A., and Larsson K. (1981) Reorganization and orientation of regenerating nerve fibers, permeurinm and epineurium in preformed mesothelial tubes—an experimental study on the sciatic nerve of rats. J. Neurosci. Res 6, 265–281.

    Article  PubMed  CAS  Google Scholar 

  • Lundborg G, Longo F.M., and Varon S. (1982). Nerve regeneration model and trophic factors in vivo. Brain Res. 232, 157–161.

    Article  PubMed  CAS  Google Scholar 

  • Lundborg G., Dahlin L.B., and Danielsen N. (1991) Ulnar nerve repair by the silicone chamber techruque. Scand. J. Plast. Reconstr. Hand Surg. 25, 79–82.

    Article  CAS  Google Scholar 

  • MacKinnon S.E. and Dellon A. L. (1990) A study of nerve regeneration across synthetic (Maxon) and biologic (collagen) nerve conduits for nerve gaps up to 5 cm in the primate. J. Reconstr. Microsurg 6, 117–121.

    Article  PubMed  CAS  Google Scholar 

  • Madison R., DaSilva CF., Dikkes P., Chin T-H., and Sidman R.L. (1985) Increased rate of peripheral nerve regeneration using bioresorbable nerve guides and laminin containing gel. Exp. Neurol. 88, 767–772.

    Article  PubMed  CAS  Google Scholar 

  • Manthorpe M., Rudge J., and Varon S. (1986) Astroglial cell contributions to neuronal survival and neurotic growth, in Astrocytes (Fedoroff S. and Vernadakrs A., eds.) vol. 2, Academic Press, San Diego, pp. 315–376.

    Google Scholar 

  • Manthorpe M., Ray J., Pettmann B., and Varon S. (1989) Ciliary neuronotrophic factors, in Nerve Growth Factors (Rush R., ed.) John Wiley & Sons, New York, pp. 31–56.

    Google Scholar 

  • Manthorpe M., Muir D., Hagg T., Engvall E., and Varon S. (1990a) Glial cell laminin and neurite outgrowth, in Differentiation and Functions of Glial Cells (Levi G., ed.) Alan Liss, Inc., New York, pp. 135–146.

    Google Scholar 

  • Manthorpe M., Muir D., Hagg T., and Varon S. (1990b) Growth promoting and inhibiting factors for neurons, in Advances in Neural Regeneration Research: The Third Infernational Neural Regeneration Research Symposium (Sell F., ed.) Alan R. Liss, New York, pp. 87–102.

    Google Scholar 

  • Manthorpe M., Engvall E., Varon S., and Muir D. (1990c) Merosm, a lamininlike extracellular matrix molecule stimulates neurite outgrowth. Soc. Neurosci. Abstr. 16, 314.

    Google Scholar 

  • Manthorpe M., Louis J. C., Hagg T., and Varon S. (1992) The ciliary neuronotrophic factor, in Neurotrophic Factors (Fallon J., and Loughlin S., ed.) Academic Press, San Diego, pp. 443–474.

    Google Scholar 

  • Molander H., Olsson Y., Engkvist O., Bowald S., and Eriksson I. (1982) Regeneration of peripheral nerve through a polyglactin tube. Muscle Nerve 5, 5407.

    Article  Google Scholar 

  • Muir D., Engvall E., Varon S., and Manthorpe M. (1989) Schwannoma cellderived inhibitor of the neurite-promoting activity of laminin. J. Cell Biol. 109, 2353–2362.

    Article  PubMed  CAS  Google Scholar 

  • Nreto-Sampedro M., Manthorpe M., Barbin G., Varon S., and Cotman C. W. (1983) Injury-induced neuronotrophic activity in the adult rat brain, Correlation with survival of delayed implants in a wound cavity. J. Neurosci. 3, 2219–2229.

    Google Scholar 

  • Pettmann B., Manthorpe M., Powell J.A., and Varon S. (1988) Biological activities of nerve growth factor bound to nitrocellulose paper by Western blotting. J. Neuroscz. 8, 3624–3632.

    CAS  Google Scholar 

  • Rende M., Hagg T., Manthorpe M., and Varon S. (1992) Nerve growth factor receptor immunoreactivity in neurons of the normal adult rat spinal cord and its modulation after peripheral nerve lesions. J. Comp. Neural. 319, 285–298.

    Article  CAS  Google Scholar 

  • Rich K. M., Alexander T. D., Pryor J.C., and Hollowell J. P. (1989) Nerve growth factor enhances regeneration through silicone chambers. Exp. Neural. 105, 162–170.

    Article  CAS  Google Scholar 

  • Rodriguez T. A., Jeffrey P. L., Thoenen H., and Barde Y. A. (1989) The survival of chick retinal ganglion cells in response to brain-derived neurotrophic factor depends on their embryonic age. Dev. Biol. 136, 296–303.

    Article  Google Scholar 

  • Schwab M.E. (1990a) Myelm-associated inhibitors of neurite growth. Exp. Neurol. 109, 2–5.

    Article  PubMed  CAS  Google Scholar 

  • Silver J. and Ogawa M.Y. (1983) Postnatally induced formation of the corpus callosum in acallosal mice on glia-coated cellulose bridges. Science 220, 1067–1069.

    Article  PubMed  CAS  Google Scholar 

  • Snow D. M., Lemmon V., Carrino D. A., Caplan A.I., and Silver J. (1990) Sulfated proteoglycans in astroglial barriers inhibit neurite outgrowth in vitro. Exp. Neurol. 109, 111–130.

    Article  PubMed  CAS  Google Scholar 

  • Taninchi M., Clark H. B., Schweitzer J.B., and Johnson E. M. (1988) Expression of nerve growth factor receptors by Schwann cells of axotomized peripheral nerves: ultrastructural location, suppression by axonal contact, and binding properties. J, Neurosci. 8, 664–681.

    Google Scholar 

  • Tuszynski M. H., Buzsaki G., and Gage F. H. (1990) Nerve growth factor infusions combined with fetal hippocampal grafts enhance reconstruction of the lesioned septohrppocampal projection. Neuroscience 36, 33–44.

    Article  PubMed  CAS  Google Scholar 

  • Vahlsing H. L., Hagg T., Spencer M., Conner J. M., Manthorpe M., and Varon S. (1991) Dose-dependent responses to nerve growth factor by adult rat cholinergic medial septum and neostriatum neurons. Bruin Res. 552, 320–329.

    Article  CAS  Google Scholar 

  • Valentini R. F., Aebischer P., Winn S.R., and Galletti P. M. (1987) Collagenand laminin-containing gels impede peripheral nerve regeneration through semipermeable nerve guidance channels. Exp. Neural. 98, 350–356.

    Article  CAS  Google Scholar 

  • Varon S., Hagg T., Vahlsing H.L., and Manthorpe M. (1991). Peripheral nerve bridges for central nervous system regeneration. Cells and Muterials 1, 83–91.

    Google Scholar 

  • Walter J., Kern-Veits B., Huf J., Stolze B., and Bonhoeffer F. (1987a) Recognition of position-specific properties of tectal cell membrane by retinal axons in vitro. Development 101, 685–96.

    PubMed  CAS  Google Scholar 

  • Walter J., Henke-Fahle S., and Bonhoeffer F. (19871b) Avoidance of posterior tectal membranes by temporal retinal axons. Development 101, 909–913.

    PubMed  CAS  Google Scholar 

  • Wendt J. S., Fagg G.E., and Cotman C. W. (1983) Regeneration of rat hippocampal fimbria fibers after fimbria transection and peripheral nerve or fetal hippocampal implantation. Exp. Neural. 79, 452–461.

    Article  CAS  Google Scholar 

  • Williams L.R. (1987) Exogenous fibrin matrix precursors stimulate the temporal progress of nerve regeneration within a silicone chamber. Neurochem Res. 12, 851–860.

    Article  PubMed  CAS  Google Scholar 

  • Williams L.R. and Varon S. (1985) Modification of fibrin matrix formation in situ enhances nerve regeneration in silicone chambers, J. Comp. Neural. 231, 209–220.

    Article  CAS  Google Scholar 

  • Williams L.R., Longo KM, Powell HC, Lundborg G., and Varon S. (1983). Spatial-temporal progress of peripheral nerve regeneration within a silicone chamber: Parameters for a bioassay. J. Camp. Neural. 218, 460–470.

    Article  CAS  Google Scholar 

  • Williams L. R., Varon S., Peterson G. M., Wictorin K., Fischer W., Bjorklund A., and Gage F. H. (1986) Continuous infusion of nerve growth factor prevents basal forebrain neuronal death after fimbria fomix transection. Proc. Natl. Acad. Sci. USA 83, 9231–9235.

    Article  PubMed  CAS  Google Scholar 

  • Wood S. J., Pritchard J., and Sofroniew M. V. (1990) Reexpression of nerve growth factor receptor after axonal injury recapitulates a developmental event in motor neurons: differential regulation when regeneration is allowed or prevented. Eur. J Neurosci. 2, 650–657.

    Article  PubMed  CAS  Google Scholar 

  • Yamamoto S.T., Iwasaki Y.,Yamamoto H., Konno H., and Isemura M. (1988) Intraneuronal laminin-like molecule in the central nervous system: demonstration of its unique differential distribution. J. Neural. Sci. 84, 1–13.

    Article  CAS  Google Scholar 

  • Yoshii S.T., Yamamuro S.I., and Hayashi M. (1987) In vivo guidance of regenerating nerve by laminin-coated filaments. Exp. Neurol. 96, 469–473.

    Article  PubMed  CAS  Google Scholar 

  • Zhou F.C. and Azmitia E. C. (1988) Laminin facilitates and guides fiber growth of transplanted neurons in adult brain, J. Chem. Neurounat. 1, 133–146.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, University of California, San Diego, La Jolla, CA

    Siluio Varon & Theo Hagg

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  1. Siluio Varon
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  2. Theo Hagg
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  1. University of Saskatchewan, Saskatoon, Canada, Edmonton, Canada

    Alan A. Boulton  & Glen B. Baker  & 

  2. University of Southern California, Los Angeles, California

    Franz Hefti

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Varon, S., Hagg, T. (1993). Models to Evaluate Effects of Neurotrophic Factors on Axonal Regeneration. In: Boulton, A.A., Baker, G.B., Hefti, F. (eds) Neurotrophic Factors. Neuromethods, vol 25. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-249-3:371

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  • DOI: https://doi.org/10.1385/0-89603-249-3:371

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-249-1

  • Online ISBN: 978-1-59259-630-0

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