Protocol

Protocols in Molecular Parasitology

Volume 21 of the series Methods in Molecular Biology™ pp 169-189

The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification

  • Margaret A. HughesAffiliated withWolfson Unit of Molecular Genetics, Liverpool School of Tropical Medicine
  • , Julian M. CramptonAffiliated withWolfson Unit of Molecular Genetics, Liverpool School of Tropical Medicine

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Abstract

The use of DNA probes for the detection and identification of para sites has become a routine practice in many laboratories and the tech nique is rapidly becoming established as a diagnostic tool for field and clinical studies. DNA probes have been used in the identification of a large number of parasites including Leishmania (14), malaria parasites (58), trypanosomes (910), Onchocerca (1113), schisto- somes (1415) and filarial parasites (1618). Undoubtedly, DNA probes will continue to make an important contribution to research progress in this area.