Protocols in Molecular Parasitology pp 169-189

Part of the Methods in Molecular Biology™ book series (MIMB, volume 21) | Cite as

The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification

  • Margaret A. Hughes
  • Julian M. Crampton

Abstract

The use of DNA probes for the detection and identification of para sites has become a routine practice in many laboratories and the tech nique is rapidly becoming established as a diagnostic tool for field and clinical studies. DNA probes have been used in the identification of a large number of parasites including Leishmania (1, 2, 3, 4), malaria parasites (5, 6, 7, 8), trypanosomes (9, 10), Onchocerca (11, 12, 13), schisto- somes (14, 15) and filarial parasites (16, 17, 18). Undoubtedly, DNA probes will continue to make an important contribution to research progress in this area.

References

  1. 1.
    Barker, D.C. and Butcher, J. (1983) The use of DNA probes in the identification of Leishmania mexicana and Leishmania braziliensis complexes. Trans.R. Soc Trop Med Hyg 77, 285–297PubMedCrossRefGoogle Scholar
  2. 2.
    Barker, D C, Gibson, L.J, Kennedy, W. P K, Nasser, A. A, and Williams, R. H (1986) The potential of using recombinant DNA species specific probes for identification of Leishmania. Parasitology 92, S139–S174.PubMedGoogle Scholar
  3. 3.
    Van Eys, G. J. J M, Schoone, G. J., Ligthart, G S., Alvar, J, Evans, D A, and Terpstra, W J (1989) Identification of Old World Leishmania by DNA recombinant probes. Mol. Biochem. Parasitol 34, 53–62.PubMedCrossRefGoogle Scholar
  4. 4.
    Howard, M K, Kelly, J. M, Lane, R. P., and Miles, M. A. (1981) A sensitive repetitive DNA probe that is specific to the Leishmania donovani complex and its use as an epidemiological and diagnostic reagent. Mol. Biochem. Parasitol. 44, 63–72.CrossRefGoogle Scholar
  5. 5.
    Oquendo, P., Goman, M., Mackay, M., Langsley, G, Walliker, D., and Scaife, J. (1986) Characterization of a repetitive sequence from the malaria parasite Plasmodium falciparum. Mol Biochem Parasitol. 18, 89–101.CrossRefGoogle Scholar
  6. 6.
    Zolg, J W. Andrade, L E, and Scott, E D (1987) Detection of Plasmodium falciparum using repetitive DNA clones as species-specific probes Mol.Biochem. Parasitol. 22, 145–151.PubMedCrossRefGoogle Scholar
  7. 7.
    Rolf, W. A, Boreham, R. E, Tapchaisn, P, Khusmith, S, Healey, A., Upcroft, P, Thararany, S, and Kidson, C. (1990) Diagnosis of Plasmodium vivax using a specific deoxyribonucleic acid probe. Trans. R Soc. Trop. Med Hyg. 84, 630–634.CrossRefGoogle Scholar
  8. 8.
    Vinyakosol, S., Snounou, G., and Brown, K N (1989) The use of a DNA probe for the differentiation of rodent malaria strains and species. Mol. Biochem.Parasitol. 32, 93–100CrossRefGoogle Scholar
  9. 9.
    Ole Moi Yoi, O K. (1987) Trypanosome species-specific DNA probes to detect infection in Tsetse flies. Parasitol. Today 3, 371–374.CrossRefGoogle Scholar
  10. 10.
    Masiga, D K. and Gibson, W. K. (1990) Specific probes for Trypanosoma evansi based on kinetoplast DNA minicircles. Mol. Biochem. Parasitol. 40, 279–284.PubMedCrossRefGoogle Scholar
  11. 11.
    Erttmann, K. D, Unnason, T R, Greene, B. M, Albiez, E. J, Boateng, J,Denke, A M, Ferraroni, J J, Karam, M, Schul-Key, H., and Williams, P. N. (1987) A DNA probe sequence specific for forest form Onchocerca volvulus. Parasitol Today 3, 377–378CrossRefGoogle Scholar
  12. 12.
    Harnett, W., Chambers, A. E., Renz, A., and Parkhouse, R. M. E. (1989) Anoligonucleotide probe specific for Onchocerca volvulus. Mol Biochem Parasitol. 35, 119–126PubMedCrossRefGoogle Scholar
  13. 13.
    Murray, K. A., Post, R J, Crampton, J. M., McCall, P J, and Kouyate, B.(1988) Cloning and characterization of a species-specific DNA sequence from Onchocerca armillata Mol. Biochem Parasitol. 30, 209–216.PubMedCrossRefGoogle Scholar
  14. 14.
    Walkev, T K, Rollinson, D, and Simpson, A. J. G (1989) A DNA probe for Schistosoma mansoni allows rapid determination of the sex of larval parasites. Mol Biochem Parasitol 33, 93–100CrossRefGoogle Scholar
  15. 15.
    Hamburger, J, Turekski, T, Kapeller, I., and Deresiewicz, C. Z (1991) Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognised by species-specific probe. Mol. Biochem. Parasitol. 44, 73–80PubMedCrossRefGoogle Scholar
  16. 16.
    Sims, B. K. L., Piessen, W F., and Wirth, D. F (1986) A DNA probe cloned into Escherichia coli for the identification of Brugia malayi. Mol Biochem. Parasitol. 19, 117–123CrossRefGoogle Scholar
  17. 17.
    McReynolds, L. A., De Simone, S. M., and Williams, S. A (1986) Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugiamalayi and the animal parasite Brugia pahangi. Proc. Natl. Acad Sci. USA 83, 797–801.PubMedCrossRefGoogle Scholar
  18. 18.
    Dissanayake, S and Piessens, W F. (1990) Cloning and characterization of Wuchereria bancrofu specific DNA sequence. Mol Biochem. Parasitol. 39, 147–150.PubMedCrossRefGoogle Scholar
  19. 19.
    Jelinek, A. J. and Schmid, C W (1982) Repetitive DNA sequences Ann. Review Biochem. 51, 813–844.CrossRefGoogle Scholar
  20. 20.
    Jeffreys, A. J., Wilson, V, and Thein, S. L. (1985) Hypervanable “mini satellite ” regions in human DNA. Nature 314, 67–73PubMedCrossRefGoogle Scholar
  21. 21.
    Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis J Mol. Biol 98, 503–517PubMedCrossRefGoogle Scholar
  22. 22.
    Hughes, M. A., Hommel, M., and Crampton, J. M (1990) The use of biotin labeled synthetic oligomers for the detection and identification of Plasmodium falciparum. Parasitology 100, 383–387PubMedCrossRefGoogle Scholar
  23. 23.
    Schwartz, D C, and Cantor, C R, (1984) Separation of yeast sized DNAs by pulsed field gradient gel electrophoresis Cell 67, 67–75CrossRefGoogle Scholar
  24. 24.
    Chu, G., Vollrath, D and Davies, R. W. (1986) Separation of large DNA molecules by contour clamped homogenous electric fields. Science 234, 1582–1585PubMedCrossRefGoogle Scholar
  25. 25.
    Le Blanc, S. M, Korman, S. H., and van der Ploeg, L. H. T. (1991) Frequent rearrangements of rRNA-encoding chromosomes in Giardia lamblia Nucleic Acids Res. 19, 4405–4412.CrossRefGoogle Scholar
  26. 26.
    Samaras, N. and Spithill, T. W (1987) Molecular karyotype of five species of Leishmania and analysis of gene locations and chromosomal rearrangements Mol. Biochem. Parasitol. 25, 279–291.PubMedCrossRefGoogle Scholar
  27. 27.
    Vierra, J. and Messing, J. (1982) The pUC plasmids, an M13mp7-denved system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259–268.CrossRefGoogle Scholar
  28. 28.
    Cohen, S. N., Chang, A. C. Y., and Hou, L. (1972) Non chromosomal antibiotic resistance in bacteria. Genetic transformation of Escherichia coli by R factor DNA. Proc Natl. Acad. Sci USA 69, 2110–2114.PubMedCrossRefGoogle Scholar
  29. 29.
    Kushner, S. R. (1978) An improved method for transformation of Escherichiacoli with ColEl derived plasmids, in Genetic Engineering: Proceedings of the International Symposium on Genetic Engineering, Milan. (Boyer, H. W and Nicosia, S., eds), Elsevier, Amsterdam, 17.Google Scholar
  30. 30.
    Dower, W J., Miller, J. F, and Ragsdale, C W. (1988) High efficiency transformation of E. coli by high voltage electroporation. Nucl. Acids Res. 16, 6127.PubMedCrossRefGoogle Scholar
  31. 31.
    Sambrook, J., Fritsch, G. F., and Maniatis, T., eds. (1989) Molecular Cloning: Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  32. 32.
    Bergen, S. L and Kimmel, A. R., eds. (1982) Methods in Enzymology vol. 152 Guide to Molecular Cloning Techniques. Academic, New York.Google Scholar
  33. 33.
    Grunstein, M. E and Hogness, D S (1975) Colony hybridization a method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA 72, 3961–3965.CrossRefGoogle Scholar
  34. 34.
    Rigby, P. W. J., Dieckmann, M., Rhodes, C, and Berg, P. (1977) Labeling deoxyribonucleic acid to high specificity in vitro by nick translation with DNA polymerases. J Mol. Biol. 113, 237–251PubMedCrossRefGoogle Scholar
  35. 35.
    Feinberg, A P and Vogelstein, B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13.PubMedCrossRefGoogle Scholar
  36. 36.
    Feinberg, A P. and Vogelstein, B. (1984) A technique for radiolabeling DNA restriction fragments to high specific activity. Anal. Biochem. 137, 266–267.PubMedCrossRefGoogle Scholar
  37. 37.
    Birnboim, D C. and Doly, J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA Nucl. Acids Res. 7, 1513–1523.PubMedCrossRefGoogle Scholar
  38. 38.
    Ish-Horowicz, D and Burke, J F (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9, 2989–2998.PubMedCrossRefGoogle Scholar
  39. 39.
    Langer, P R., Waldrop, A A., and Ward, D. C. (1981) Enzymatic synthesis of biotin labeled polynucleotides-Novel nucleic acid affinity probes Proc. Natl Acad Sci. USA 78, 6633–6637.PubMedCrossRefGoogle Scholar
  40. 40.
    Leary, J. J., Brigati, D. J., and Ward, D. C. (1983) Rapid and sensitive colorimetnc method for visualizing biotin labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bioblots. Proc. Natl. Acad. Sci. USA 80, 4045–4049.PubMedCrossRefGoogle Scholar
  41. 41.
    Forster, A. C, Mclnnes, J. L, Skingle, D. C, and Symons, R. H (1985) Nonradioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent photobiotin. Nucl Acids Res. 13, 745–761PubMedCrossRefGoogle Scholar
  42. 42.
    Murasugi, A. and Wallace, B. R. (1984) Biotin labeled oligonucleotides. Enzymatic syntheses and use as hybridization probes DNA 3, 269–277Google Scholar
  43. 43.
    Boehringer Mannheim applications manual (1989) DNA Labelling and Detection. Nonradioactive. Boehringer Mannheim, Mannheim, GermanyGoogle Scholar
  44. 44.
    Matthews, J. A., Batki, A., Hynds, C, and Kricka, L. J. (1985) Enhanced chemiluminescent method for the detection of DNA in dot blot assays. Anal. Biochem. 151, 205–209PubMedCrossRefGoogle Scholar
  45. 45.
    Matthews, J. A. and Kricka, L J. (1988) Analytical strategies for the use of DNA probes Anal. Biochem 169, 1–25.PubMedCrossRefGoogle Scholar
  46. 46.
    Lund, V., Lindquist, B M, and Eggset, G. (1989) Detection of specific DNA sequences using antibodies recognizing UV labeled DNA. Nucl. Acids Res. 17, 539–551.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc, Totowa, NJ 1993

Authors and Affiliations

  • Margaret A. Hughes
    • 1
  • Julian M. Crampton
    • 1
  1. 1.Wolfson Unit of Molecular GeneticsLiverpool School of Tropical MedicineLiverpoolUK

Personalised recommendations