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Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1)

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Enzymes of Molecular Biology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 16))

Abstract

From Chapter 1 on nucleases, we know that the term DNase refers to an enzyme that endonucleolytically cleaves DNA molecules. This chapter deals with those DNases that preferentially catalyze the hydrolysis of double-stranded DNA (ds DNA) and that have found a use in the various techniques employed in molecular biology, The Type II restriction endonucleases obviously fall into this category, however they are a large subject on their own and therefore are dealt with in a separate section of this book. DNases acting preferentially on single-stranded DNA (ss DNA) substrates are also dealt with in another section of this chapter.

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© 1993 Humana Press Inc.

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Weir, A.F. (1993). Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1). In: Burrell, M.M. (eds) Enzymes of Molecular Biology. Methods in Molecular Biology™, vol 16. Humana Press. https://doi.org/10.1385/0-89603-234-5:7

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  • DOI: https://doi.org/10.1385/0-89603-234-5:7

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-322-1

  • Online ISBN: 978-1-59259-503-7

  • eBook Packages: Springer Protocols

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