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Pronase (EC 3.4.24.4)

  • Patricia J. Sweeney
  • John M. Walker
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 16)

Abstract

Pronase is the name given to a group of proteolytic enzymes that are produced in the culture supernatant of Streptomyces griseus K-1 (1, 2, 3). Pronase is known to contain at least ten proteolytic components: five serine-type proteases, two Zn2+ endopeptidases, two Zn2+-leucine aminopeptidases, and one Zn2+ carboxypeptidase (4,5). Pronase therefore has very broad specificity, and is used in cases where extensive or complete degradation of protein is required. It has been used, for example, to reveal the protein components of cell organelles by the hydrolysis of tissue slices (6), and as an alternative to proteinase K to remove protein during plasmid DNA (7), chromosomal DNA (8), and RNA isolation (9, 10, 11). Another use of pronase is the production of a protein hydrolysate suitable for amino acid analysis (12,13).

Keywords

Amino Acid Analysis Protein Hydrolysate Broad Specificity Sodium Carbonate Solution Leucine Aminopeptidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Patricia J. Sweeney
    • 1
  • John M. Walker
    • 1
  1. 1.Division of Biological SciencesUniversity of HertfordshireHatfieldUK

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