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Taq Polymerase (EC 2.7.7.7)

With Particular Emphasis on Its Use in PCR Protocols

  • Protocol
Enzymes of Molecular Biology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 16))

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Abstract

Taq polymerase (EC 2.7.7.7) is a thermostable DNA-dependent DNA polymerase that was first isolated in 1976 from Thermus aquaticus strain YT-1 (ATTC # 25 104)(1). It catalyzes the template-directed polymerization of dNTPs at high temperatures.

When Taq polymerase was isolated in 1976, nobody could have known the impact the application of Taq polymerase to the polymerase chain reaction (PCR) would have on molecular biology (2,3). In an issue of Science in 1989, the enzyme was dubbed molecule of the year (4).

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  1. Medizinische Hochschule Hannover, Zentrum Biochemie, Abteilung Biophysikalische Chemie, Hannover, Germany

    Axel Landgraf & Heiner Wolfes

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  1. Axel Landgraf
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  2. Heiner Wolfes
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  1. Advanced Technologies (Cambridge) Limited, Cambridge, UK

    Michael M. Burrell

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Landgraf, A., Wolfes, H. (1993). Taq Polymerase (EC 2.7.7.7). In: Burrell, M.M. (eds) Enzymes of Molecular Biology. Methods in Molecular Biology™, vol 16. Humana Press. https://doi.org/10.1385/0-89603-234-5:31

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  • DOI: https://doi.org/10.1385/0-89603-234-5:31

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