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Identification and Analysis of Function of Heterotrimeric Guanine Nucleotide-Binding Proteins Expressed in Neural Tissue

  • Protocol
Book cover Intracellular Messengers

Part of the book series: Neuromethods ((NM,volume 20))

Abstract

Most neurotransmitters, hormones, and growth factors that cause alterations in the rate of synthesis of intracellular messengers interact with cell surface receptors that are members of a family of single polypeptide proteins. Secondary and tertiary structure predictions for such receptors indicate that they are glycoproteins in which highly hydrophobic blocks of amino acids produce seven trans-plasma membrane-spanning elements. In all known cases, such receptors are required to interact with members of a family of heterotrimeric guanine nucleotide-binding proteins (G proteins) before agonist-induced alterations in the enzymatic activity of intracellular messenger-generating systems can be detected.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Glasgow, Glasgow, Scotland

    Graeme Milligan

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  1. Graeme Milligan
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Editor information

Editors and Affiliations

  1. University of Saskatchewan, Saskatoon, Canada

    Alan A. Boulton

  2. University of Alberta, Edmonton, Canada

    Glen B. Baker

  3. University of Cambridge, Cambridge, UK

    Colin W. Taylor

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© 1992 The Humana Press Inc.

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Milligan, G. (1992). Identification and Analysis of Function of Heterotrimeric Guanine Nucleotide-Binding Proteins Expressed in Neural Tissue. In: Boulton, A.A., Baker, G.B., Taylor, C.W. (eds) Intracellular Messengers. Neuromethods, vol 20. Humana Press. https://doi.org/10.1385/0-89603-207-8:1

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  • DOI: https://doi.org/10.1385/0-89603-207-8:1

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-207-1

  • Online ISBN: 978-1-59259-625-6

  • eBook Packages: Springer Protocols

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