Abstract
The major advantage in the use of avidin-biotin technology for isolation purposes is that an improved capacity for purification of an antigen often results (1,2). The antibody, bound to the column via an avidin-biotin bridge, is less affected by the chemistry of immobilization or by physical interactions (hydrophobic, electrostatic, salting-out effects, precipitation, and so on) with the solid support. Such potential deleterious effects are borne by avidin, which is a highly stable protein, and thus serves as a physicochemical buffer for the antibody.
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References
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© 1992 The Humana Press, Inc., Totowa, NJ
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Bayer, E.A., Wilchek, M. (1992). Immunochemical Applications of Avidin-Biotin Technology. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:149
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DOI: https://doi.org/10.1385/0-89603-204-3:149
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
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