Abstract
The second step required in the application of avidin-biotin technology is to prepare an appropriate avidin-associated probe or probes for the desired application (for general reviews, see refs. 1 and 2). For example, a fluorescent form of avidin can be used for fluorescence microscopy, fluorescence-activated cell sorting, and in some cases, for immunoassay. Like-wise, an avidin-enzyme conjugate can be used for immunoblotting, immunoassay, light microscopy, and in some cases, electron microscopy. An immobilized form of avidin can be used for isolation purposes (see Note 1).
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References
Wilchek, M. and Bayer, E. A., eds. (1990) Avidin-Biotin Technology. Methods in Enzymology, vol. 184, Academic, Orlando, FL.
Wilchek, M. and Bayer, E. A. (1988) The avidin-biotin complex in bioanalytical applications. Anal. Biochem. 171, 1–32.
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© 1992 The Humana Press, Inc., Totowa, NJ
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Bayer, E.A., Wilchek, M. (1992). Avidin–Biotin Technology. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:143
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DOI: https://doi.org/10.1385/0-89603-204-3:143
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
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