Abstract
Batch culture involves the inoculation of a known volume or mass of cells into a volume of (normally) defined medium. Growth is allowed to proceed, and the resulting biomass is harvested at some stage during the growth cycle. Throughout the growth period, no additions are made to the media, with the exception of acid and alkali, if needed, for pH control. The scale of operation depends on the requirements.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bailey, J. E. and Ollis, D. F. (1986) Biochemical Engineering Fundamentals, 2nd Ed.(McGraw-Hill, Singapore.)
Morris, P. (1986) Regulation of product synthesis in cell cultures of Catharunthus Rosen II comparison of production media. Planta Medica 2, 121–126.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Humana Press Inc.
About this protocol
Cite this protocol
Bond, P.A. (1990). Batch Suspension Culture. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:495
Download citation
DOI: https://doi.org/10.1385/0-89603-161-6:495
Publisher Name: Humana Press
Print ISBN: 978-0-89603-161-6
Online ISBN: 978-1-59259-493-1
eBook Packages: Springer Protocols