Plant Cell and Tissue Culture pp 467-475 | Cite as
Selection of Chloroplast Mutants
Protocol
- 8 Citations
- 1.8k Downloads
Abstract
The chloroplast genome encodes a number of proteins, including thylakoid proteins and the large subunit of ribulose biphosphate carboxylase, associated with the structure and function of the chloroplast (1-2). In addition, many components of the chloroplast translational machinery, such as all of the RNAs and some of the ribosomal proteins, are coded by the chloroplast DNA. Although there have been numerous investigations into the genetics of algal chloroplasts, similar studies with higher plants have been hampered by the uniparental (maternal) pattern of transmission of chloroplasts observed in most species, and the shortage of suitable genetic markers (3,4).
Keywords
Shoot Culture Streptomycin Sulfate Protoplast Culture Leaf Strip Albino Mutant
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
- 1.Dyer, T.A.(1985)The chloroplast genome and its products, in Oxford Surveys ofPlant Molecular and Ceil Biology,vol2 (Miflin, B. J., ed.), Oxford University Press, New York, pp. 147–177.Google Scholar
- 2.Borner, T.and Sears, B. B. (1986) Plastome Mutants. PIant Molecular Biology Reporfer 4, 69–92.CrossRefGoogle Scholar
- 3.Maliga, P. (1986) Cell fusion to introduce genetic information coded by chloroplasts and mitochondria in flowering plants, in Molecular Development Biology Liss, New York, pp. 45–53.Google Scholar
- 4.Maliga, P. Breznovits, A.,and Márton, L. (1973) Streptomycin resistant plants from haploid callus culture of tobacco. Nature (New Biol.)244, 28–30.CrossRefGoogle Scholar
- 5.Galun, E.and Aviv, D. (1983) Cytoplasmic hybridization: Genetic and breeding applications, in Handbook of Plant Cell Culture, vol 1 (Evans, D. A., Sharp, W. R., Ammirata, I’. V.,and Yamada, Y., eds.), Macmillan, New York, pp. 358–392.Google Scholar
- 6.Pelletier, G. (1986) Plant organelle genetics through somatic hybridization in Oxford Suroeys ofPlant MoZecularand Cell Biology, vol3 (Miflin, B. J., ed.), Oxford University Press, pp. 96–121.Google Scholar
- 7.Medgyesy, P., Fejes, E.,and Maliga, P. (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. PYOC. Natl. Acad. Sci. USA 82, 6960–6964.CrossRefGoogle Scholar
- 8.Svab, Z.and Maliga, P. (1986) Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor. AppZ. Gent. 72, 637–643.CrossRefGoogle Scholar
- 9.Cs¬olÖ A.and Maliga, P. (1984) Large scale isolation of maternally inherited lincomycin resistance mutations in diploid Nicotiana plumbuginifolia protoplast cultures. Mol. Gen. Genet. 196, 407–412.CrossRefGoogle Scholar
- 10.Cs¬olÖ, A., Medgyesy, P., Hideg, E., Demeter, S., Márton, L., and Maliga, P. (1985) Triazine resistant Nicotiana mutants from photomixotrophic cell cultures. Mol. Gen. Genet. 200, 508–510.CrossRefGoogle Scholar
- 11.Fluhr, R., Aviv, D., Galun, E., and Edelman, M. (1985) Efficient induction and selection of chloroplast-encodedanitbiotic resitant mutantsin Nicotiana. Proc. Nutl. Acad. Sci. USA 82, 1485–1489.CrossRefGoogle Scholar
- 12.Hagemann, R. (1982) Induction of plastome mutations by nitroso-urea-compounds, in Methods in Chloroplust Molecular Biology (Edelman, M., Hallick, R. B., and Chua, N. H., eds.), Elsevier Biomedical Press, Amsterdam, pp. 119–127.Google Scholar
- 13.Hosticka, L. P. and Hanson, M. R. (1984) Induction of plastid mutations in tomatoes by nitrosomethylurea. J. Hered. 75, 242–246.Google Scholar
- 14.Maliga, P. (1984) Cell culture procedures for mutant selection and characterization in Nicotiunu plumbaginifolia, in Cell Culture and Somatic Cell Genetics of Plants, vol1(Vasil, I. K., ed.), Academic, New York, pp. 552–562.Google Scholar
Copyright information
© Humana Press Inc. 1990