Abstract
The culture of fruit tissues as whole organs or isolated tissue sections has been conducted with various species (1). Whole, isolated ovaries have been successfully cultured to give rise to mature fruits (e.g., strawberry). Typically, however, when an isolated portion of the fruit tissue is introduced into a sterile environment, it immediately loses structural integrity and degenerates into a rapidly dividing callus mass (2). Loss of structural integrity is correspondingly associated with an alteration of physiology that is subsequently reflected in the production of an altered metabolism. Therefore, a meaningful study of fruit development using callus derived from fruit tissues is often not possible. Recently, we studied the parameters involved in the maintenance of citrus fruit tissue integrity (2). In this paper, the culture of isolated fruit tissues, as well as half and whole fruit culture, is demonstrated using the lemon fruit (Fig. 1–3).
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References
Nitsch, J.P. (1963) The in vitro culture of flowers and fruits, in Plant Tissue and Organ Culture-A Symposium (Maheshwari, P. and Rangaswamy, N. S., eds.), Univ. of Delhi, Delhi, pp. 198–214.
Tisserat, B. and Galletta, P. D. (dy1987) In vitro culture of lemon juice vesicles. Plant Cell Tissue and Organ Culture 11,81–95.
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© 1990 Humana Press Inc.
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Tisserat, B., Galletta, P.D., Jones, D. (1990). Fruit Organ Cultures. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:121
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DOI: https://doi.org/10.1385/0-89603-161-6:121
Publisher Name: Humana Press
Print ISBN: 978-0-89603-161-6
Online ISBN: 978-1-59259-493-1
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