Establishment of Lymphoblastoid Cell Lines

  • A. Doyle
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 5)

Abstract

The ability to establish long-term B lymphocyte cultures from patients carrying particular genetic characteristics or with the ability to secrete specific antibodies (1) is an extremely valuable technique. However, there are several basic principles to follow in the approach to this technology. It is the purpose of this chapter to provide all the information necessary to run an efficient and safe Epstein-Barr Virus (EBV) transformation system. More detailed information regarding the use of this technique for the preparation of human monoclonal antibodies is given elsewhere (2).

Keywords

Human Monoclonal Antibody Conical Centrifuge Tube Mononuclear Cell Fraction Dilute Blood Sample Liquid Nitrogen Container 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Steinitz, M., Klein, G., Koskimies, S., and Makel, O. (1977) EB virus-induced B lymphocyte cell lines producing specific antibody. Nature 269, 420.PubMedCrossRefGoogle Scholar
  2. 2.
    Crawford, D. H. (1986) Use of the virus to prepare human-derived monoclonal antibodies, in The Epstein-Barr Virus: Recent Advances (Epstein, M. A. and Achong, B. G., eds.), William Heinemann Medical Books, London, p. 249.Google Scholar
  3. 3.
    Adams, A. (1975) EBV Production, Concentration and Purification (Ablushi, D. V., Aalesed, H. G., and De The, G., eds.), IARC, Lyon, France, p. 129.Google Scholar
  4. 4.
    Miller, G. and Lipman, M. (1973) Release of infectious Epstein-Barr virus by transformed marmoset leucocytes. Proc. Natl. Acad. Sci.USA 70, 190.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1990

Authors and Affiliations

  • A. Doyle
    • 1
  1. 1.European Collection of Animal Cell CulturesPHLS CAMRSalisburyUK

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