Establishment of Lymphoblastoid Cell Lines
The ability to establish long-term B lymphocyte cultures from patients carrying particular genetic characteristics or with the ability to secrete specific antibodies (1) is an extremely valuable technique. However, there are several basic principles to follow in the approach to this technology. It is the purpose of this chapter to provide all the information necessary to run an efficient and safe Epstein-Barr Virus (EBV) transformation system. More detailed information regarding the use of this technique for the preparation of human monoclonal antibodies is given elsewhere (2).
KeywordsHuman Monoclonal Antibody Conical Centrifuge Tube Mononuclear Cell Fraction Dilute Blood Sample Liquid Nitrogen Container
- 2.Crawford, D. H. (1986) Use of the virus to prepare human-derived monoclonal antibodies, in The Epstein-Barr Virus: Recent Advances (Epstein, M. A. and Achong, B. G., eds.), William Heinemann Medical Books, London, p. 249.Google Scholar
- 3.Adams, A. (1975) EBV Production, Concentration and Purification (Ablushi, D. V., Aalesed, H. G., and De The, G., eds.), IARC, Lyon, France, p. 129.Google Scholar