Abstract
Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking the gel with rabbit antimouse immunoglobulin before staining with Coomassie Blue.
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Reference
Gustafsson, B. and Holme, T. (1983) Monoclonal antibodies against group-and type-specific lipopolysaccharide antigens of Vibrio cholerae O:1. J. Clin. Microbiol. 18, 480–485.
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© 1990 The Humana Press Inc.
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Gustafsson, B. (1990). Isoelectric Focusing and Immunofixation of Monoclonal Antibodies. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:627
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DOI: https://doi.org/10.1385/0-89603-150-0:627
Publisher Name: Humana Press
Print ISBN: 978-0-89603-150-0
Online ISBN: 978-1-59259-492-4
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