Abstract
Many research workers and clinicians working in routine laboratories are now using the “tools” developed by molecular biologists over the past 10 years to produce DNA sequences that can be used as probes to detect specific genes. Thus it is possible to screen food for salmonella (1) or other organisms in a test much more quickly than conventional methods involving culturing the organisms. The presence of certain types of human papillomavirus in cervical smears can be used as an indicationof neoplasia (2). DNA probes can also be used to screen antenatally for diseases such as sickle cells anemia (3) or β°-thalassemia (4).
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References
Fitts, R., Diamond, M., and Hamilton, C. (1983) DNA-DNA hybridisation assay for detection of Salmonella spp. in foods. Appl. Environ. Microbiol. 46, 1146–1151.
Wickenden, C., Malcolm, A.D.B., Steele, A., and Coleman, D.V. (1985) Screening for wart virus infection in normal and abnormal cervices by DNA hybridisation of cervical scrapes. Lancet i, 65–67.
Orkin, S.H., Little, P.F.R., Kazazian, H.H., and Bochm, C.D. (1982) Improved detection of the sickle mutation by DNA analysis. N. Eng. J. Med. 307, 32–36.
Renz, M. and Kurz, C. (1984) A colorimetric method for DNA hybridisation. Nucleic Acids Res. 12, 3435–3444.
Denhardt, D. (1966) A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23, 641–645.
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© 1988 The Humana Press Inc.
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Woodhead, J.L., Fallon, R., Figueiredo, H., Malcolm, A.D.B. (1988). Enzyme-Labeled DNA Probes. In: Walker, J.M. (eds) New Nucleic Acid Techniques. Methods in Molecular Biology, vol 4. Humana Press. https://doi.org/10.1385/0-89603-127-6:415
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DOI: https://doi.org/10.1385/0-89603-127-6:415
Publisher Name: Humana Press
Print ISBN: 978-0-89603-127-2
Online ISBN: 978-1-59259-491-7
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