New Protein Techniques pp 269-298 | Cite as
Computer Analysis of 2-D Electrophoresis Gels
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Abstract
Although two-dimensional (2-D) gel electrophoresis has been used for the past ten years, a large amount of potentially useful information is still generally unused, since accurate and rapid methods for extracting, managing, and analyzing the vast quantity of data that can be derived from the gels are not widely available. The difficulty is partly caused by the large number of spots as well as artifacts present in a 2-D gel and the non-uniformity in the electrophoresis process that causes distorted spot patterns and makes comparison among gels difficult. With the advent of data base management systems (DBMS) and computer graphics, 2-D gel data can be organized and manipulated with relative ease. This chapter describes work that has been done toward automating the process of extracting useful quantitative information from 2-D gels and comparing spot data derived from separate gels. The design and implementation of a data base and a data analysis system for 2-D gels are also outlined.
Keywords
Protein Spot Central Core Spot Data Major Spot Data Base Management SystemReferences
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