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Monoamine Methyltransferases

Phenylethanolamine N-Methyltransferase and Catechol O-Methyltransferase

  • Protocol
Neurotransmitter Enzymes

Part of the book series: Neuromethods ((NM,volume 5))

Abstract

Phenylethanolamine N-methyltransferase (PNMT) (EC 2.1.1.28, S-adenosyl-L-methionine: phenylethanolamine N-methyltransferase) is the enzyme that catalyzes the biosynthesis of norepinephrine [noradrenaline; (NA)] to epinephrine [adrenaline; (A)] in the presence of S-adenosyl-L-methionine (SAM) as the methyl donor. The enzyme is localized mainly in the adrenal medulla and in small amounts in discrete brain regions containing A cell bodies and nerve terminals. Adrenal PNMT has been extensively purified and well characterized, but the brain enzyme has not been well studied, although many similarities between them have been observed.

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References

  • Anderson P J. and D’Ioro A. (1968) Purification and properties of catechol-O-methyltransferase. Biochem. Pharmacol. 17, 1943–1949

    PubMed  CAS  Google Scholar 

  • Assicot M and Bohuon C (1969) Production of antibodies to catechol-O-methyltransferase (EC 2.1.1 6 ) of rat liver. Biochem Pharmacol. 18, 1893–1898.

    PubMed  CAS  Google Scholar 

  • Assicot M. and Bohuon C. (1970) Purification and studies of catechol-O-methyltransferase of rat liver Eur J Biochem. 12, 490–495.

    PubMed  CAS  Google Scholar 

  • Axelrod J. (1962) Purification and properties of phenylethanolamine N-methyltransferase. J. Biol Chem. 237, 1657–1660

    PubMed  CAS  Google Scholar 

  • Axelrod J. and Laroche M. J (1959) Inhibitor of O-methylation of epinephrine and norepinephrine in vitro and in vivo. Science 130, 800.

    PubMed  CAS  Google Scholar 

  • Axelrod J. and Tomchick R. (1958) Enzymatic O-methylation of epinephrine and other catechols J Blol Chem. 233, 702–705.

    CAS  Google Scholar 

  • Axelrod J. and Vesell E S. (1970) Heterogeneity of N-and O-methyltransferase Mol Pharmacol. 6, 78–84.

    PubMed  CAS  Google Scholar 

  • Axelrod J,, Albers W., and Clemente C. D. (1959) Distribution of catechol-O-methyltransferase in the nervous system and other tissues J Neurochem. 5, 68–72

    CAS  Google Scholar 

  • Bade P., Christ W., Rakow D, and Coper H. (1976) Comparison of catechol-O-methyltransferase from rat brain, erythrocytes and liver Life Sci 19, 1833–1844.

    PubMed  CAS  Google Scholar 

  • Baetge E E., Moon H M., Kaplan B B, Park D H., Reis D. J, and Joh T H. (1983) Identification of clones containing DNA complementary to phenylethanolamine N-methyltransferase mRNA Neurochem. Intl. 5, 611–617

    CAS  Google Scholar 

  • Bakke O. M. (1970) O-methylation of simple phenols in the rat Acta Pharmacol. Toxicol. 28, 28–38

    CAS  Google Scholar 

  • Ball P., Knuppen R., and Breuer H. (1971) Purification and properties of a catechol-O-methyltransferase of human liver Eur J Biochem. 21, 517–525.

    PubMed  CAS  Google Scholar 

  • Baron M, Gruen R., Levitt M., Hunter C, and Asms L. (1984) Erythrocyte catechol O-methyltransferase activity in schizophrenia: Analysis of family data Am J Psychiatry 141, 29–32

    PubMed  CAS  Google Scholar 

  • Belleau B. and Burba J (1963) Occupancy of adrenergic receptors and inhibition of catechol O-methyltransferase by tropolones J Med Chem 6, 755–759

    PubMed  CAS  Google Scholar 

  • Berenbeim D. M., Wong D. L., Masover S. J, and Ciaranello R D (1979) Regulation of synthesis and degradation of rat adrenal phenylethanolamine N-methyltransferase. III Stabilization of PNMT against thermal and tryptic degradation by S-adenosylmethionine. Mol. Pharmacol. 16, 482–490

    PubMed  CAS  Google Scholar 

  • Borchardt R T. (1981) Catechol O-Methyltransferase in Methods in Enzymology (Jakoby W. B., ed), vol 77, pp 267–272, Academic, New York.

    Google Scholar 

  • Borchardt R T. and Cheng C F (1978) Purification and characterization of rat heart and brain catechol methyltransferase Biochem. Biophys. Acta 522, 49–62.

    PubMed  CAS  Google Scholar 

  • Borchardt R. T, Cheng C. F., Cooke P H., and Creveling C. R. (1974) The purification and kinetic properties of liver microsomal-catechol O-methyltransferase Life Sci. 14, 1089–1100

    PubMed  CAS  Google Scholar 

  • Borchardt R. T, Cheng C. F., and Thakker D R. (1975) Purification of catechol O-methyltransferase by affinity chromatography. Biochem Biophys. Res. Commun. 63, 69–77.

    PubMed  CAS  Google Scholar 

  • Borchardt R. T, Huber J A, and Houston M. (1982) Catechol-O-methyltransferase. 10 5-substituted 3-hydroxy-4-methoxybenzoic acid (isovanillic acids) and 5-substituted 3-hydroxy-4-methoxybenz-aldehydes (isovanillins) as potential inhibitors J Med Chem. 25, 258–271

    PubMed  CAS  Google Scholar 

  • Briggs M. H. and Briggs M. (1973) Hormonal Influences on erythrocyte catechol-O-methyl transferase activity in humans. Experientia 29, 278–280.

    PubMed  CAS  Google Scholar 

  • Caspers M L. (1982) Inhibition by lead of phenylethanolamine N-methyltransferase. Biochem. Pharmacol. 31, 1985–1988

    PubMed  CAS  Google Scholar 

  • Chen C. H., Klein D. C, and Robinson J. C (1974) Catechol-O-methyltransferase in rat placenta, human placenta and chonocarcinoma grown in culture. J. Reprod. Fertil 39, 407–410.

    PubMed  CAS  Google Scholar 

  • Ciaranello R. D, (1978a) Regulation of phenylethanolamine N-methyltransferase synthesis and degradation. I. Regulation by rat adrenal glucocorticoids. Mol. Pharmacol 14, 478–489.

    PubMed  CAS  Google Scholar 

  • Ciaranello R. D (1978b) Regulation of phenylethanolamine N-methyltransferase Biochem. Pharmacol. 27, 1895–1897.

    PubMed  CAS  Google Scholar 

  • Claranello R D., Barchas R. E, Byers G. S, Stemmle D. W, and Barchas J. D. (1969) Enzymatic synthesis of adrenaline in mammalian brain. Nature 221, 368–369.

    Google Scholar 

  • Ciaranello R. D., Wong D. L., and Berenbeim D. M, (1978) Regulation of phenylethanolamine N-methyltransferase synthesis and degradation. II. Control of the thermal stability of the enzyme by an endogenous stabilizing factor Mol Pharmacol. 14, 490–501.

    PubMed  CAS  Google Scholar 

  • Cohn C K., Dunner D L., and Axelrod J. (1970) Reduced catechol-O-methyltransferase activity in red blood cells of women with primary affective disorder. Science 170, 1323–1324.

    PubMed  CAS  Google Scholar 

  • Connett R. J. and Kirshner N. (1970) Purification and properties of bovine phenylethanolamine N-methyltransferase. J. Blol. Ckem. 245, 329–334.

    CAS  Google Scholar 

  • Coward J. K, Slisz E P, and Wu F Y. H. (1973) Kinetic studies on catechol O-methyltransferase Product inhibition and the nature of the catechol binding site. Biochemistry, 12, 2291–2297.

    PubMed  CAS  Google Scholar 

  • Creveling C R., Morris N, Shimizu H, Ong H. H., and Daly J. (1972) Catechol O-methyltransferase. IV. Factors affecting m-and p-methylation of substituted catechols. Mol. Pharmacol. 8, 398–409.

    PubMed  CAS  Google Scholar 

  • Creveling C R., Borchardt R. T, and Isersky C. (1973) Immunological Characterization of Catechol-O-Methyltransferase, in Frontiers in Catecholamine Research (Usdin E. and Snyder S. H., eds.), pp.117–119, Pergamon, New York.

    Google Scholar 

  • Davidson J. R. T., McLeod M. N., Turnbull C. D., White H. L., and Feuer E J. (1979) Catechol-O-methyltransferase activity and classification of depression. Biol Psychiatry 14, 937–942.

    PubMed  CAS  Google Scholar 

  • Deguchi T. and Barchas J. (1971) Inhibition of transmethylations of biogenic amines by S-adenosylhomocysteine. Enhancement of transmethylation by adenosylhomocysteinase J. Biol. Chem. 246, 3175–3181.

    PubMed  CAS  Google Scholar 

  • Diaz-Borges J. M, Urbina M., and Drqan B. D. (1978) Some properties of phenylethanolamine N-methyltransferase of rat brain. Neurochem Res. 3, 15–26.

    PubMed  CAS  Google Scholar 

  • Dunner D L. (1979) The use of erythrocyte catechol-O-methyltransferase activity as a possible marker for vulnerability of affective disorder. Psychopharmacol. Bull. 15, 32

    PubMed  CAS  Google Scholar 

  • Dunner D. L, Cohn C. K., Gershon E S., and Goodwin F. K. (1971) Differential catechol-O-methyltransferase activity in unipolar and bipolar affective illness Arch. Gen. Psychiatry 25, 348–353.

    PubMed  CAS  Google Scholar 

  • Dunner D. L, Levitt M., Kumbaraci T., and Fieve R. R. (1977) Erythrocyte catechol-O-methyltransferase in primary affective disorder. Biol Psychiatry 12, 237–244.

    Google Scholar 

  • Ebstein R., Belmaker R H., Benbenisty D, and Rimon R. (1976) Electrophoretic pattern of red blood cell catechol-O-methyltransferase in schizophrenia and manic-depressive illness. Biol. Psychiatry 11, 613–623.

    PubMed  CAS  Google Scholar 

  • Fahn S., Comi R, Snider S R., and Prasad A L. N (1979) Effect of a catechol-O-methyltransferase inhibitor, U-0521, with levo-DOPA admmistrauon Biochem. Pharmacol. 28, 1221–1225.

    PubMed  CAS  Google Scholar 

  • Floderus Y., Book J. A, and Wetterberg L. (1981) Erythrocyte catechol-O-methyltransferase activity in related families with schizophrenia. Clin Genet 19, 379–385.

    PubMed  CAS  Google Scholar 

  • Flohe L. (1974) Catechol-O-methyltransferase. Int Pharmacopsychiatry 9, 52–60.

    PubMed  CAS  Google Scholar 

  • Flohe L. and Schwabe K. P (1970) Kinetics of purified catechol-O-methyltransferase. Biochim. Biophys. Acta 220, 469–476.

    PubMed  CAS  Google Scholar 

  • Fuller R. W. (1982) Pharmacology of brain epinephrine neurons. Ann Rev. Pharmacol. Toxicol. 22, 31–55.

    CAS  Google Scholar 

  • Fuller R. W. and Hemrick S. K (1979) Properties of norepinephrine N-methyltransferase from rat, cat, and chicken brain. Comp Biochem. Physiol 62C, 243–245.

    CAS  Google Scholar 

  • Fuller R. W. and Hemrick-Luecke S. K. (1983) Species differences in epinephrine concentration and norepinephrine N-methyltransferase activity in hypothalamus and brain stem Comp. Biochem Physiol. 74C, 47–49.

    CAS  Google Scholar 

  • Fuller R. W. and Hunt J. M (1967) Inhibition of phenylethanolamine N-methyltransferase by its product, epinephrine. Life Sci. 6, 1107–1112.

    PubMed  CAS  Google Scholar 

  • Fuller R. W. and Molloy B. B. (1977) Inhibition in vitro of norepinephrine N-methyltransferase by 2-aminotetralins, analogs of phenylethyl-amines with rigid conformation. Biochem Pharmacol 26, 446–447.

    PubMed  CAS  Google Scholar 

  • Fuller R. W., Warren B. J, and Molloy B. B. (1970) Substrate specificity of phenylethanolamine N-methyltransferase from rabbit adrenal. Biochim. Biophys Acta 222, 210–212.

    PubMed  CAS  Google Scholar 

  • Fuller R. W., Mills J., and Marsh M. M (1971) Inhibition of phenethanolamine N-methyltransferase by ring-substituted α-methyl-phenethylamines (amphetamines) J Med Chem 14, 322–325

    PubMed  CAS  Google Scholar 

  • Fuller R. W., Hemrick, S K., and Molloy B. B (1977) Position of a chloro substituent on phenylethanolamine as a determinant of methylation by rabbit adrenal norepinephrine N-methyltransferase. Res Commun. Chem. Pathol. Pharmacol 18, 577–580.

    PubMed  CAS  Google Scholar 

  • Gershon E. S. and Jonas W Z (1975) Erythrocyte soluble catechol-O-methyltransferase activity in primary affective disorder. A clinical and genetic study Arch Gen Psychiatry 32, 1351–1356

    PubMed  CAS  Google Scholar 

  • Gershon E. S., Lake C. R., and Leckman J F. (1979) Reduced Erythrocyte COMT and Plasma DBH Activities not Associated With Affective Disorders in Patients and Relatives, in Catecholamines: Basic and Clinical Frontiers (Usdin E., Kopin I. J., and Barchas J., eds.), pp, 1934–1936, Pergamon, New York

    Google Scholar 

  • Gershon E. S., Goldin L. R, Lake C. R, Murphy D. L., and Guroff J. J. (1980) Genetics of Plasma Dopamine-β-Hydroxylase (DBH), Erythrocyte Catechol-O-Methyltransferase (COMT), and Platelet Monoamine Oxidase (MAO) in Pedigrees of Patients With Affective Disorders, in Enzymes and Neurotransmitters in Mental Disease (Usdin E., Sourkes T. L., and Youdim M B H., eds.), pp 281–299, John Wiley, New York

    Google Scholar 

  • Gordonsmith R. H, Raxworthy M. J., and Gulliver P A. (1982) Substrate stereospecificity and selectivity of catechol-O-methyltransferase for DOPA, DOPA derivatives and α-substituted catecholamines. Biochem Pharmacol 31, 433–437.

    PubMed  CAS  Google Scholar 

  • Groshong R, Baldessarini R. J, Gibson D A., Lipinski J. F, Axelrod D, and Pope A (1978) Activities of type A and B MAO and catechol-O-methyltransferase in blood cells and skin fibroblasts of normal and chronic schizophrenic subjects Arch. Gen Psychiatry 35, 1198–1205

    PubMed  CAS  Google Scholar 

  • Grunewald G. L., Grindel J. M, Vincek W. C., and Borchardt R. T. (1975) Importance of the aromatic ring in adrenergic amines: Nonaromatic analogues of phenylethanolamine as substrates for phenylethanol-amine N-methyltransferase Mol Pharmacol. 11, 694–699

    PubMed  CAS  Google Scholar 

  • Guldberg H C and Marsden C A (1975) Catechol-O-methyl transferase: Pharmacological aspects and physiological role. Pharmacol Rev. 27, 135–206

    PubMed  CAS  Google Scholar 

  • Gulliver P. A, and Tipton K F (1978a) Direct extraction radioassay for catechol-O-methyl-transferase activity. Biochem Pharmacol 27, 773–775

    PubMed  CAS  Google Scholar 

  • Gulliver P A. and Tipton K F (1978b) The purification and properties of pig-liver catechol-O-methyl transferase. Eur. J. Biochem. 88, 439–444.

    PubMed  CAS  Google Scholar 

  • Gulliver P A and Tipton K F (1979) The purification and properties of pig brain catechol-O-methyltransferase J Neurochem. 32, 1525–1529.

    PubMed  CAS  Google Scholar 

  • Hoffman A. R., Ciaranello R D, and Axelrod J. (1975) Substrate and inhibitor kinetics of bovine phenylethanolamine N-methyltransferase. Biochem Pharmacol 24, 544–546

    PubMed  CAS  Google Scholar 

  • Hokfelt T, Fuxe K., Goldstein M, and Johansson O. (1974) Immunohistochemical evidence for the existence of adrenaline neurons in the rat brain. Brain Res 66, 235–251.

    CAS  Google Scholar 

  • Huh M. M. O. and Friedhoff A. J. (1979) Multiple molecular forms of catechol-O-methyltransferase: Evidence for two distinct forms, and their purification and physical characterization. J. Biol. Chem. 254, 299–308

    PubMed  CAS  Google Scholar 

  • Joh T. H and Goldstein M (1973) Isolation and characterization of multiple forms of phenylethanolamine N-methyltransferase. Mol. Pharmacol. 9, 117–129

    PubMed  CAS  Google Scholar 

  • Kaplan G. P., Hartman B K., and Creveling C. R (1980) Anti-catechol-O-methyltransferase: Demonstration of specificity and immunological cross-reactivity with the enzyme from rat liver, kidney, brain and choroid plexuses Neurochem. Res. 5, 869–877

    PubMed  CAS  Google Scholar 

  • Kety S. S. (1967) Current biochemical approaches to schizophrenia N Engl. J. Med. 276, 325–331

    PubMed  CAS  Google Scholar 

  • Kirshner N. and Goodall McC. (1957) The formation of adrenaline from noradrenaline. Biochim. Biophys. Acta 24, 658–659

    PubMed  CAS  Google Scholar 

  • Kitabchi A E and Williams R. H. (1969) Phenylethanolamine N-methyltransferase in human adrenal gland Biochim Biophys Acta 178, 181–184.

    PubMed  CAS  Google Scholar 

  • Kitabchi A E., Solomon S S., and Williams R. H. (1968) Stimulatory effects of insulin and glucagon on phenylethanolamine N-methyltransferase of rat adrenal. Proc Soc. Exp Biol. Med. 127, 296–300.

    PubMed  CAS  Google Scholar 

  • Knuppen V R, Holler M., Tilmann D., and Breuer H. (1969) Interactions between oestrogens and catecholamines. II Effect of oestrogen on the degradation and methylation of adrenaline in mouse under in vivo conditions. Hoppe-Seylers J. Physiol Chem 350, 1301–1309.

    CAS  Google Scholar 

  • Kondo Y., Nagatsu I, Karasawa N., Kojima H, and Nagatsu T. (1982) Immunohistocytochemical and biochemical studies on catecholamine-synthesizing enzymes of the lumbar sympathetic ganglia and sciatic nerves of bullfrogs. Biomed Res. 3, 197–206.

    CAS  Google Scholar 

  • Krakoff L R. and Axelrod J. (1967) InhibitIon of phenylethanolamine N-methyltransferase. Biochem. Pharmacol. 16, 1384–1386.

    PubMed  CAS  Google Scholar 

  • Lee H. S., Schulz A. R., and Fuller R. W. (1974) Isolation of multiple forms of rabbit adrenal norepinephrine N-methyltransferase Arch Biochem. Biophys. 163, 735–741

    PubMed  CAS  Google Scholar 

  • Lee H. S., Schulz A. R., and Fuller R. W. (1978) Isolation and purification of rabbit adrenal norepinephrine N-methyltransferase isozyme. Arch. Biochem. Biophys. 185, 222–227.

    PubMed  CAS  Google Scholar 

  • Lew J. Y., Miyamoto T, and Goldstein M. (1976) Inhibition of PNMT activity in the adrenal glands and brainstem of rats. Biochem. Pharmacol. 25, 1433–1434.

    CAS  Google Scholar 

  • Lew J. Y., Matsumoto Y., Pearson J, Goldstein M, Hokfelt T., and Fuxe K. (1977) Localization and characterization of phenylethanolamine N-methyltransferase in the brain of various mammalian species Brain Res. 119, 199–210.

    PubMed  CAS  Google Scholar 

  • Lewander T., Pongracz G. V, Backstrom M, and Wetterberg L (1981) Dopamine metabolism in red blood cells in schizophrenia Clin Genet. 19, 410–413

    PubMed  CAS  Google Scholar 

  • Liang N. Y, Tessel R E., Grunewald G L., and Borchardt R. T. (1982) Inhibitors of phenylethanolamine N-methyltransferase. 1. Effects of 2-cyclooctyl-2-hydroxyethylamine on rat brain and adrenal catechol-amine content and blood pressure. J Pharmacol. Exp. Ther 223, 375–381

    PubMed  CAS  Google Scholar 

  • Masover S. J., Berenbeim D. M., and Ciaranello R. D. (1979) Regulation of synthesis and degradation of rat adrenal phenylethanolamine N-methyltransferase. IV. Synergistic stabilization of the enzyme against thermal and tryptic degration by S-adenosylmethionine and biogenic amine substrates. Mol. Pharmacol 16, 491–503

    PubMed  CAS  Google Scholar 

  • Matthysse S. and Baldessarini R. J. (1972) S-adenosylmethionine and catechol-O-methyltransferase in schizophrenia. Am. J. Psychiatry 128, 1310–1312.

    PubMed  CAS  Google Scholar 

  • McGeer P. L and McGeer E. G. (1964) Formation of adrenaline by brain tissue. Biochem. Biophys. Res. Commun. 17, 502–507.

    CAS  Google Scholar 

  • Molinoff P. B and Axelrod J. (1971) Biochemistry of catecholamines. Ann Rev. Biochem. 40, 465–499.

    PubMed  CAS  Google Scholar 

  • Molinoff P. B., Brimijoin S, Weinshilboum R., and Axelrod J. (1970) Neurally mediated increase in dopamine-β-hydroxylase activity. Proc. Natl. Acad Sci. USA 66, 453–458.

    PubMed  CAS  Google Scholar 

  • Park D. H and Joh T H. (1985) Species-specific charge forms of phenylethanolamine N-methyltransferase Brain Res., in press.

    Google Scholar 

  • Park D H. and Joh T. H (1984) Activation of adrenal phenylethanolamine N-methyltransferase by phosphate. Biochem Pharmacol. 33, 2911–2913.

    PubMed  CAS  Google Scholar 

  • Park D. H., Baetge E. E, Kaplan B B., Albert V. R, Reis D. J, and Joh T. H. (1982) Different forms of adrenal phenylethanolamine N-methyltransferase Species-specific posttranslational modification. J. Neurochem 38, 410–414.

    PubMed  CAS  Google Scholar 

  • Pendleton R. G, Kaiser C., and Gessner G. (1976) Studies on adrenal phenylethanolamine N-methyltransferase (PNMT) with SKF 64139, a selective inhibitor J Pharmacol. Exp Ther. 197, 623–632.

    PubMed  CAS  Google Scholar 

  • Pendleton R G., Gessner G, Weiner G, Jenkins B., Sawyer J., Bondinell W., and Intoccia A (1979) Studies on SKF 29661, an organ-specific inhibitor of phenylethanolamine N-methyltransferase. J. Pharmacol. Exp. Ther 208, 24–30.

    PubMed  CAS  Google Scholar 

  • Pohorecky L A. and Baliga B S (1973) Purification and properties of rat adrenal phenylethanolamine N-methyl transferase. Arch. Biochem. Biophys 156, 703–711.

    PubMed  CAS  Google Scholar 

  • Pohorecky L. A. and Wurtman R. J. (1971) Adrenocortical control of epinephrine synthesis. Pharmacol. Rev. 23, 1–35.

    PubMed  CAS  Google Scholar 

  • Pohorecky L. A., Zigmond M, Karten H., and Wurtman R. J. (1969) Enzymatic conversion of norepinephrine to epinephrine by the brain. J Pharmacol Exp Ther. 165, 190–195.

    PubMed  CAS  Google Scholar 

  • Pohorecky L A., Baliga B S., Wurtman R. J., and Bartter F C (1973) Adrenocortical control of catecholamine metabolism in the dog adrenal medulla. Relationship to protein synthesis. Endocrinology 93, 566–574.

    PubMed  CAS  Google Scholar 

  • Poitou P., Assicot M., and Bohuon C. (1974) Soluble and Membrane catechol-O-methyltransferase in red blood cells of schizophrenic patients. Biomedicine 21, 91–93.

    PubMed  CAS  Google Scholar 

  • Rafferty M. F. and Grunewald G. L. (1982) The remarkable substrate activity for phenylethanolamine N-methyltransferase of some conformationally defined phenylethylamines lacking a side-chain hydroxyl group. Mol. Pharmacol 22, 127–132

    PubMed  CAS  Google Scholar 

  • Raxworthy M. J., Gulliver P. A., and Hughes P J (1982) The cellular location of catechol-O-methyltransferase in rat liver. Naunyn Schmiedebergs Arch Pharmacol 320, 182–188.

    PubMed  CAS  Google Scholar 

  • Rivett A. J. and Roth J. A. (1982) Kinetic studies on the O-methylation of dopamine by human brain membrane-bound catechol O-methyltransferase Biochemistry 21, 1740–1742.

    PubMed  CAS  Google Scholar 

  • Rivett A. J., Francis A, and Roth J A. (1983a) Distinct cellular localization of membrane-bound and soluble forms of catechol-O-methyltransferase in brain. J Neurochem 40, 215–219

    PubMed  CAS  Google Scholar 

  • Rivett A. J., Francis A., and Roth J. A. (1983b) Localization of membrane-bound catechol-O-methyltransferase. J. Neurochem 40, 1494–1496.

    PubMed  CAS  Google Scholar 

  • Roth J A. (1980) Presence of membrane-bound catechol-O-methyltransferase in human brain. Biochem. Pharmacol 29, 3119–3122.

    PubMed  CAS  Google Scholar 

  • Saavedra J. M., Palkovits M., Brownstein M J., and Axelrod J (1974) Localization of phenylethanolamine N-methyltransferase in the rat brain nuclei. Nature 248, 695–696.

    PubMed  CAS  Google Scholar 

  • Saavedra J. M., Brownstein M. J., and Palkovits M. (1976) Distribution of catechol-O-methyltransferase, histamine N-methyltransferase and monoamine oxidase in specific areas of the rat brain Brain Res. 118, 152–156

    PubMed  CAS  Google Scholar 

  • Senoh S., Daly J, Axelrod J., and Witkop B. (1959) Enzymatic p-O-methylation by catechol O-methyl transferase J Am. Chem Soc. 81, 6240–6245.

    CAS  Google Scholar 

  • Shopsin B., Wilk S., Gershon S., Roffman M, and Goldstein M. (1973) Collaborative Psychopharmacologic Studies Exploring Catecholamine Metabolism in Psychiatric Disorders, in Frontiers in Catecholamine Research (Usdin E. and Snyder S. H., eds.), Pergamon, New York.

    Google Scholar 

  • Teitelman G, Skaper S, Baker H, Park D. H., Joh T. H., and Adler R. (1984) Expression of phenylethanolamine N-methyltransferase in sympathetic neurons and extraadrenal chromaffin tissue of chick embryos in vivo and in vitro. Dev. Brain Res 13, 283–291.

    CAS  Google Scholar 

  • Thoenen H., Mueller R A., and Axelrod J. (1970) Neuronally dependent induction of phenylethanolamine N-methyltransferase by 6-hydroxydopamine. Biochem Pharmacol. 19, 669–673.

    PubMed  CAS  Google Scholar 

  • Tunnicliff G and Ngo T T (1983) Kinetics of rat brain soluble catechol-O-methyltransferase and its inhibition by substrate analogues. Int J Biochem. 15, 733–738.

    PubMed  CAS  Google Scholar 

  • Vogel W. H., Lewis L. E., and Boehme D. H (1976) Phenylethanolamine N-methyltransferase activity in various areas of human brain, tissues and fluids. Brain Res. 115, 357–359.

    PubMed  CAS  Google Scholar 

  • White H L. and Wu J. C. (1975) Properties of catechol O-methyltransferases from brain and liver of rat and human. Biochem. J. 145, 135–143.

    PubMed  CAS  Google Scholar 

  • White H. L, McLeod M. N, and Davidson J. R. T. (1976) Catechol O-methyltransferase in red blood cells of schizophrenic, depressed and normal human subjects, Br J. Psychiatry 128, 184–187.

    PubMed  CAS  Google Scholar 

  • Wurtman R. J and Axelrod J. (1966) Control of enzymatic synthesis of adrenaline in the adrenal medulla by adrenal cortical steroids. J Biol. Chem. 241, 2301–2305.

    PubMed  CAS  Google Scholar 

  • Wurtman R. J., Axelrod J,, Vesell E. S., and Ross G. T. (1968) Species differences in inducibility of phenylethanolamine N-methyltransferase. Endocrinology 82, 584–590.

    PubMed  CAS  Google Scholar 

  • Yu P. H. (1978) Phenylethanolamine N-methyltransferase from the brain and adrenal medulla of the rat. A comparison of their properties. Neurochem. Res 3, 755–762

    PubMed  CAS  Google Scholar 

  • Zurcher G. and Da Prada M. (1982) Rapid and sensitive single-step radiochemical assay for catechol-O-methyltransferase. J Neurochem 38, 191–195.

    PubMed  CAS  Google Scholar 

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  1. Laboratory of Neurobiology, Cornell University Medical College, New York, New York

    Dong H. Park

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Alan A. Boulton Glen B. Baker Peter H. Yu

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© 1986 The Humana Press Inc.

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Park, D.H. (1986). Monoamine Methyltransferases. In: Boulton, A.A., Baker, G.B., Yu, P.H. (eds) Neurotransmitter Enzymes. Neuromethods, vol 5. Humana Press. https://doi.org/10.1385/0-89603-079-2:117

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  • DOI: https://doi.org/10.1385/0-89603-079-2:117

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-079-4

  • Online ISBN: 978-1-59259-610-2

  • eBook Packages: Springer Protocols

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