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The Isolation of High Molecular Weight Eukaryotic DNA

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2))

Abstract

The isolation of high molecular weight eukaryotic DNA in good yield is an important prerequisite for the analysis of specific sequences by Southern blotting (Chapter 9), or for molecular cloning in phage or cosmid vectors (Chapter 49).

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References

  1. Kunkel, L. M., Smith, K. D., Boyer, S. H., Borgaonkar, D. S., Wachtel, S. S., Miller, O. J., Breg, W. R., Jones, H. W., and Rory, J. M. (1977) Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc. Natl. Acad. Sci. USA 74, 1245–1249.

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  2. Old, J. M., and Higgs, D. R. (1983) Gene Analysis. In Methods in Hematology. The Thalassaemias (ed. Weatherall, D. J.), p. 78. Butler & Tanner, Rome and London.

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  3. Maniatis, T., Fritsch, E. R, and Sambrook, J. (1982) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory, New York.

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Authors and Affiliations

Authors

Editor information

John M. Walker

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© 1984 The Humana Press Inc.

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Mathew, C.G.P. (1984). The Isolation of High Molecular Weight Eukaryotic DNA. In: Walker, J.M. (eds) Nucleic Acids. Methods in Molecular Biology, vol 2. Humana Press. https://doi.org/10.1385/0-89603-064-4:31

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  • DOI: https://doi.org/10.1385/0-89603-064-4:31

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-064-0

  • Online ISBN: 978-1-59259-489-4

  • eBook Packages: Springer Protocols

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