Abstract
Several methods are available for recovering proteins electrophoretically from polyacrylamide gels directly after electrophoresis or after staining for protein (see, for example, Chapter 19). Fluorography (see Chapter 17) is used to detect small amounts of proteins or specifically modified forms of proteins by labeling these with specific precursors such as 3H-lysine or 3H-acetate.
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References
Mende, L. M., Waterborg, J. H., Mueller, R. D., and Matthews, H. R. (1983) Isolation, identification and characterization of the histones from plasmodia of the true slime mold, Physarum polycephalum, using extraction with guanidinium hydrochloride. Biochem. 22, 38–51.
Waterborg, J. H., and Matthews, H. R. (1983) Acetylation sites in histone H3 from Physarum polycephalum. FEBS Lett., 162, 416–419.
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© 1984 Humana Press
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Waterborg, J.H., Matthews, H.R. (1984). Recovery of Proteins from Dried Polyacrylamide Gels after Fluorography. In: Walker, J.M. (eds) Proteins. Methods in Molecular Biology™, vol 1. Humana Press. https://doi.org/10.1385/0-89603-062-8:153
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DOI: https://doi.org/10.1385/0-89603-062-8:153
Publisher Name: Humana Press
Print ISBN: 978-0-89603-062-6
Online ISBN: 978-1-59259-488-7
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