Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands

  • Ahad A. Rahim
Part of the Methods in Molecular Biology™ book series (MIMB, volume 373)

Abstract

High combinatorial phage display libraries have become an important tool in the search for ligand-receptor interactions. The advantage this approach offers is the ability to screen large repertoires of peptides, displayed on the coat proteins of bacteriophages, against a target at the same time. In addition, no prior knowledge is required of the target or the ligand. However, to characterize the peptides of interest a short length of the bacteriophage genome that encodes the peptide sequence requires DNA sequencing. The number of candidate bacteriophages can be large and so sequencing is expensive, timeconsuming, and laborious. Therefore, a methodology using Pyrosequencing® has been developed where 96-phage displaying a seven amino acid peptide can be analyzed simultaneously within 45 min and at a fraction of the cost associated with traditional automated Sanger sequencing.

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Ahad A. Rahim
    • 1
  1. 1.Gene Therapy Group, Chester Beatty LaboratoriesInstitute of Cancer ResearchLondonUK

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