Pyrosequencing® Protocols pp 1-13

Part of the Methods in Molecular Biology™ book series (MIMB, volume 373)

The History of Pyrosequencing®

  • Pål Nyrén

Abstract

One late afternoon in the beginning of January 1986, bicycling from the lab over the hill to the small village of Fullbourn, the idea for an alternative DNA sequencing technique came to my mind. The basic concept was to follow the activity of DNA polymerase during nucleotide incorporation into a DNA strand by analyzing the pyrophosphate released during the process. Today, the technique is used in multidisciplinary fields in academic, clinical, and industrial settings all over the word. The technique can be used for both single-base sequencing and whole-genome sequencing, depending on the format used.

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Pål Nyrén
    • 1
  1. 1.Department of BiotechnologyAlbaNova University CenterStockholmSweden

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