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First Analysis of Macromolecular Crystals

Biochemistry and X-Ray Diffraction
  • David Jeruzalmi
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 364)

Abstract

Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.

Key Words

Macromolecular crystals biochemical analysis of crystal contents crystal mounting X-ray diffraction crystal symmetry space groups 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • David Jeruzalmi
    • 1
  1. 1.Department of Molecular and Cellular BiologyHarvard UniversityCambridge

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