Automated Structure Solution With autoSHARP

  • Clemens Vonrhein
  • Eric Blanc
  • Pietro Roversi
  • Gérard Bricogne
Part of the Methods in Molecular Biology™ book series (MIMB, volume 364)


We present here the automated structure solution pipeline “autoSHARP.” It is built around the heavy-atom refinement and phasing program SHARP, the density modification program SOLOMON, and the ARP/wARP package for automated model building and refinement (using REFMAC). It allows fully automated structure solution, from merged reflection data to an initial model, without any user intervention. We describe and discuss the preparation of the user input, the data flow through the pipeline, and the various results obtained throughout the procedure.

Key Words

SHARP autoSHARP automation structure solution 


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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Clemens Vonrhein
    • 1
  • Eric Blanc
    • 2
  • Pietro Roversi
    • 3
  • Gérard Bricogne
    • 1
  1. 1.Global Phasing Ltd.CambridgeUK
  2. 2.European Bioinformatics InstituteWellcome Trust Genome CampusCambridgeUK
  3. 3.Department of Biochemistry, Laboratory of Molecular BiophysicsUniversity of OxfordOxford

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