Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel

  • Oren Beske
  • Daniel Bassoni
  • Simon Goldbard
Part of the Methods in Molecular Biology book series (MIMB, volume 356)


The CellCard™ system enables the analysis of multiple cell types within a single microtiter well. In doing so, the CellCard system not only determines the effect of an experimental condition on a cell type of interest, but also the relative selectivity of that response across nine other cell types. In addition, this approach of cellular multiplexing is a means of miniaturization without the necessity of microfluidic devices. The standard 96-well plate generates ten 96-well plates of data (or, the equivalent of a 960-well plate). Taken together, the CellCard technology enables multiple cell types to be assayed within a single microtiter well allowing for the simultaneous determination of cellular activity and compound selectivity. This chapter will describe a method by which multiple cell types can be simultaneously assayed for biological parameters of interest.

Key Words

Cell-based assay CellCard carrier selectivity 


  1. 1.
    Abraham, V. C., Taylor, D. L., Haskins, J. R. (2004) High content screening applied to large-scale cell biology. Trends Biotechnol. 22(1), 15–22.CrossRefGoogle Scholar
  2. 2.
    Giuliano, K. A., Haskins, J. R., Taylor, D. L. (2003) Advances in high content screening for drug discovery. Assay Drug Dev. Technol. 1(4), 565–577.CrossRefGoogle Scholar
  3. 3.
    Liptrot, C. (2001) High content screening—from cells to data to knowledge. Drug Discov. Today 6(16), 832–834.CrossRefGoogle Scholar
  4. 4.
    Arnould, R., Dubois, J., Abikhalil, F., et al. (1990) Comparison of two cytotoxicity assays—tetrazolium derivative reduction (MTT) and tritiated thymidine uptake—on three malignant mouse cell lines using chemotherapeutic agents and investigational drugs. Anticancer Res. 10(1), 145–154.Google Scholar
  5. 5.
    Rausch, O. (2005) Use of high content analysis for compound screening and target selection. IDrugs 8(7), 573–577.Google Scholar
  6. 6.
    Perlman, Z. E., Mitchison, T. J., Mayer, T. U. (2005) High content screening and profiling of drug activity in an automated centrosome-duplication assay. Chembiochem. 6(2), 218.CrossRefGoogle Scholar
  7. 7.
    Borchert, K. M., Galvin, R. J., Frolik, C. A. (2005) High content screening assay for activators of the Wnt/Fzd pathway in primary human cells. Assay Drug Dev. Technol. 3(2), 133–141.CrossRefGoogle Scholar
  8. 8.
    Vogt, A., Cooley, K. A., Brisson, M., Tarpley, M. G., Wipf, P., and Lazo, J. S. (2003) Cell-active dual specificity phosphatase inhibitors identified by high content screening. Chem. Biol. 10(8), 733–742.CrossRefGoogle Scholar
  9. 9.
    Beske, O., Guo, J., Li, J., et al. (2004) A novel encoded particle technology that enables simultaneous interrogation of multiple cell types. J. Biomol. Screen 9(3), 173–185.CrossRefGoogle Scholar
  10. 10.
    Beske, O. A. G. (2002) High-thgoughput cell analysis using multiplexed array technologies. Drug Discov. Today 7(18), S131–S135.CrossRefGoogle Scholar
  11. 11.
    Beske, O., Goldbard, S., and Turpin, P. (2005) The CellCard System; a novel approach to assessing compound selectivity for lead prioritization. Combinatorial Chemistry High-Throughput Screen 8(4), 293–299.CrossRefGoogle Scholar
  12. 12.
    Borchert, K. M., Galvin, R. J., Frolik, C. A., et al. (2005) High content screening assay for activators of the Wnt/Fzd pathway in primary human cells. Assay Drug Dev. Technol. 3(2), 133–141.CrossRefGoogle Scholar
  13. 13.
    Di Nicolantonio, F., Knight, L. A., Whitehouse, P. A., et al. (2004). The ex vivo characterization of XR5944 (MLN944) against a panel of human clinical tumor samples. Mol. Cancer Ther. 3(12), 1631–1637.Google Scholar
  14. 14.
    Terashima, M., Hayashi, K., Fukushima, M., et al. (1996). Drug sensitivity testing for clinical samples from oesophageal cancer using adhesive tumour cell culture system. Br. J. Cancer 74(1), 73–77.CrossRefGoogle Scholar

Copyright information

© Humana Press, Inc. 2007

Authors and Affiliations

  • Oren Beske
    • 1
  • Daniel Bassoni
    • 1
  • Simon Goldbard
    • 1
  1. 1.Vitra Bioscience, Inc.Mountain View

Personalised recommendations