High Content Screening pp 129-138

Part of the Methods in Molecular Biology book series (MIMB, volume 356)

Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel

  • Oren Beske
  • Daniel Bassoni
  • Simon Goldbard
Protocol

Abstract

The CellCard™ system enables the analysis of multiple cell types within a single microtiter well. In doing so, the CellCard system not only determines the effect of an experimental condition on a cell type of interest, but also the relative selectivity of that response across nine other cell types. In addition, this approach of cellular multiplexing is a means of miniaturization without the necessity of microfluidic devices. The standard 96-well plate generates ten 96-well plates of data (or, the equivalent of a 960-well plate). Taken together, the CellCard technology enables multiple cell types to be assayed within a single microtiter well allowing for the simultaneous determination of cellular activity and compound selectivity. This chapter will describe a method by which multiple cell types can be simultaneously assayed for biological parameters of interest.

Key Words

Cell-based assay CellCard carrier selectivity 

Copyright information

© Humana Press, Inc. 2007

Authors and Affiliations

  • Oren Beske
    • 1
  • Daniel Bassoni
    • 1
  • Simon Goldbard
    • 1
  1. 1.Vitra Bioscience, Inc.Mountain View

Personalised recommendations