Protein Engineering Protocols pp 23-34

Part of the Methods in Molecular Biology™ book series (MIMB, volume 352)

Global Incorporation of Unnatural Amino Acids in Escherichia coli

  • Jamie M. Bacher
  • Andrew D. Ellington

Abstract

The incorporation of amino acid analogs is becoming increasingly useful. Site-specific incorporation of unnatural amino acids allows the application of chemical biology to protein-specific investigations and applications. However, the global incorporation of unnatural amino acids allows for tests of proteomic and genetic code hypotheses. For example, the adaptation of organisms to unnatural amino acids may lead to new genetic codes. To understand and quantify changes from such perturbations, an understanding is required of the microbiological and proteomic responses to the incorporation of unnatural amino acids. Here we describe protocols to characterize the effects of such proteome-wide perturbations.

Key Words

Unnatural amino acids genetic code ambiguity amino acid misincorporation amino acylation errors amino acid analogs genetic code evolution 

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Jamie M. Bacher
    • 1
  • Andrew D. Ellington
    • 2
    • 3
  1. 1.The Skaggs Institute for Chemical BiologyThe Scripps Research InstituteLa Jolla
  2. 2.Institute for Cellular and Molecular BiologyUniversity of TexasAustin
  3. 3.Department of BiochemistryUniversity of TexasAustin

Personalised recommendations