Protocol

MicroRNA Protocols

Volume 342 of the series Methods in Molecular Biology™ pp 267-276

Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein

  • Ranhui DuanAffiliated withDepartment of Human Genetics, Emory University School of Medicine
  • , Peng JinAffiliated withDepartment of Human Genetics, Emory University School of Medicine

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Abstract

Fragile X syndrome, a common form of inherited mental retardation, is caused by the loss of the Fragile X mental retardation protein (FMRP). FMRP, which may regulate translation in neurons, not only associates with specific messenger RNAs (mRNAs) and with microRNAs (miRNAs), but also associates with the components of the miRNA pathway, including the Dicer and Argonaute proteins. It has been proposed that FMRP regulates the translation of its mRNA targets through miRNAs. In this chapter, we describe the protocol to identify the mRNAs and miRNAs associated with FMRP in vivo. The same method could also be applied to other RNA-binding proteins interacting with specific mRNAs or miRNAs.

Key Words

Fragile X syndrome FMRP immunoprecipitation messenger RNA microRNA microarray