Embryonic Stem Cell Protocols pp 265-272

Part of the Methods in Molecular Biology book series (MIMB, volume 329)

Efficient Transfer of HSV-1 Amplicon Vectors Into Embryonic Stem Cells and Their Derivatives

  • Dieter Riethmacher
  • Filip Lim
  • Thomas Schimmang

Abstract

The derivation of specialized differentiated cells from embryonic stem (ES) cells is now a major focus for future therapies involving cell and organ replacement in humans. To obtain populations of differentiated cells for transplantation into the human body, highly optimized protocols are needed that allow direction of the development of ES cells and their derivatives. These protocols mostly include the use of a combination of growth factors that control the differentiation of ES cells into highly specialized cells found in adult organs. The introduction of different growth factors into ES cells and their derivatives via viral gene transfer may greatly facilitate the optimization of these protocols. In this chapter, we describe a method based on herpes simplex virus type 1, which allows efficient gene transfer during several steps of a protocol, directed to obtain neural progenitors from ES cells. This protocol therefore may allow the study of potential factors influencing the cell fate or differentiation of ES cells and their derivatives.

Key Words

Amplicon embryonic stem cell gene transfer Herpes nestin neural progenitor 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Dieter Riethmacher
    • 1
  • Filip Lim
    • 1
  • Thomas Schimmang
    • 2
  1. 1.Center for Molecular NeurobiologyUniversity of HamburgHamburgGermany
  2. 2.Institute for Biology and Molecular GeneticsUniversity of ValladolidValladolidSpain

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