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Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 330)

Abstract

The model system of embryonic stem (ES) cell differentiation in vitro via cellular aggregates (embryoid bodies, EBs) can be used to analyze cell differentiation from a pluripotent stem cell via progenitor cells up to terminally differentiated cell types. ES cells are known to be pluripotent; they have the capacity to differentiate into any cell lineage of the three germ layers. Using various ES cell lines, we characterized chondrogenic and osteogenic differentiation in EBs by histochemical staining, immunostaining, mRNA-in situ hybridization, and reverse transcriptase polymerase chain reaction analysis. Here, we describe in detail our established protocols to analyze chondrogenic differentiation of ES cells. We summarize different ways to modulate ES cell-derived chondrogenic differentiation.

Key Words

  • Chondrogenic differentiation
  • embryonic stem cells
  • osteogenic differentiation

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© 2006 Humana Press Inc.

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Kramer, J., Hargus, G., Rohwedel, J. (2006). Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology™, vol 330. Humana Press. https://doi.org/10.1385/1-59745-036-7:171

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  • DOI: https://doi.org/10.1385/1-59745-036-7:171

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-784-6

  • Online ISBN: 978-1-59745-036-2

  • eBook Packages: Springer Protocols