The Role of Hex in Hemangioblast and Hematopoietic Development

  • Rebecca J. Chan
  • Robert Hromas
  • Mervin C. Yoder
Part of the Methods in Molecular Biology™ book series (MIMB, volume 330)


The orphan homeobox gene Hex has been shown to be critical for normal murine liver development using Hex-/- mice. Because of the early lethality of the Hex-/- mice, however, in vitro embryonic stem cell differentiation assays were employed to elucidate the role of Hex in murine hematopoiesis. These studies revealed that Hex is not required for hemangioblast development but is required in a dose-dependent fashion for hemangioblast differentiation to definitive hematopoietic progenitors. Contained here are the methods needed to conduct embryonic stem cell-derived in vitro hematopoietic assays.

Key Words

Embryonic stem cells hematopoiesis Hex hemangioblasts in vitro differentiation 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Rebecca J. Chan
    • 1
  • Robert Hromas
    • 2
  • Mervin C. Yoder
    • 1
  1. 1.Wells Center for Pediatric ResearchIndiana University School of MedicineIndianapolis
  2. 2.Cancer Research and Treatment CenterUniversity of New MexicoAlbuquerque

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