In Situ Hybridization Protocols pp 103-113

Part of the Methods in Molecular Biology™ book series (MIMB, volume 326) | Cite as

In Situ Hybridization of Whole-Mount Embryos

  • Murray Hargrave
  • Josephine Bowles
  • Peter Koopman

Summary

The ability to visualize the expression of a gene in both time and space is an essential tool of developmental biology. Here, we detail a robust method for in situ hybridization of RNA probes to whole pieces of fixed tissue. This method has been optimized for reliable and sensitive visualization of the spatial patterns of gene expression in mouse embryo tissue.

Key Words

In situ hybridization whole mount gene expression. 

References

  1. 1.
    Tuatz D. and Pfiefle C. (1989) A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98, 81–85.CrossRefGoogle Scholar
  2. 2.
    Christiansen J. H., Dennis C. L., Wicking C. A., Monkley S. J., Wilkinson D. G., and Wainwright B. J. (1995) Murine Wnt-11 and Wnt-12 have temporally and spatially restricted expression patterns during embryonic development. Mech. Dev. 51, 341–350.PubMedCrossRefGoogle Scholar
  3. 3.
    Wilkinson D. G. and Nieto M. A. (1993) Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts. Meth. Enzymol. 225, 361–373.PubMedCrossRefGoogle Scholar
  4. 4.
    Wilkinson D. G. (1992) Whole mount in situ hybridization of vertebrate embryos, in In Situ Hybridization: A Practical Approach (Wilkinson D. G., ed.), IRL Press, Oxford, UK, pp. 75–83.Google Scholar
  5. 5.
    Jowett T., Mancera M., Amores A., and Yan Y. (1996) In situ hybridization to embryo whole mounts and tissue sections: mRNA detection and application to developmental studies, in In Situ Hybridization (Clark M., ed.), Chapman and Hall, London, UK, pp. 91–121.CrossRefGoogle Scholar
  6. 6.
    Jowett T. and Lettice L. (1994) Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin-and fluorescein-labelled probes. Trends Genet. 10, 73–74.PubMedCrossRefGoogle Scholar
  7. 7.
    Hauptmann G. and Gerster T. (1994) Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos. Trends Genet. 10, 266.PubMedCrossRefGoogle Scholar
  8. 8.
    Wright E., Hargrave M. R., Christiansen J., et al. (1995) The Sry-related gene Sox-9 is expressed during chondrogenesis in mouse embryos. Nat. Genet. 9, 15–20.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Murray Hargrave
  • Josephine Bowles
  • Peter Koopman

There are no affiliations available

Personalised recommendations