In Situ Hybridization of Whole-Mount Embryos

  • Murray Hargrave
  • Josephine Bowles
  • Peter Koopman
Part of the Methods in Molecular Biology™ book series (MIMB, volume 326)


The ability to visualize the expression of a gene in both time and space is an essential tool of developmental biology. Here, we detail a robust method for in situ hybridization of RNA probes to whole pieces of fixed tissue. This method has been optimized for reliable and sensitive visualization of the spatial patterns of gene expression in mouse embryo tissue.

Key Words

In situ hybridization whole mount gene expression. 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Murray Hargrave
  • Josephine Bowles
  • Peter Koopman

There are no affiliations available

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