Advertisement

Isolation of Stromal Stem Cells From Human Adipose Tissue

  • Andrew C. Boquest
  • Aboulghassem Shahdadfar
  • Jan E. Brinchmann
  • Philippe Collas
Part of the Methods in Molecular Biology™ book series (MIMB, volume 325)

Abstract

The stromal compartment of mesenchymal tissues is thought to harbor stem cells that display extensive proliferative capacity and multilineage potential. Stromal stem cells offer a potentially large therapeutic potential in the field of regenerative medicine. Adipose tissue contains a large number of stromal stem cells, is relatively easy to obtain in large quantities, and thus constitutes a very convenient source of stromal stem cells. Importantly, the number of stem cells obtained is compatible with extensive analyses of the cells in an uncultured, freshly isolated, form. This chapter describes procedures for isolating millions of highly purified stromal stem cells from human adipose tissue and methods of establishing polyclonal and monoclonal cultures of adipose tissue-derived stem cells.

Key Words

Adipose tissue mesenchyme stem cell stroma 

References

  1. 1.
    Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., et al. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284, 143–147.CrossRefPubMedGoogle Scholar
  2. 2.
    Howell, J. C., Lee, W. H., Morrison, P., Zhong, J., Yoder, M. C., and Srour, E. F. (2003) Pluripotent stem cells identified in multiple murine tissues. Ann. N. Y. Acad. Sci. 996, 158–173.CrossRefPubMedGoogle Scholar
  3. 3.
    Arai, F., Ohneda, O., Miyamoto, T., Zhang, X. Q., and Suda, T. (2002) Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation. J. Exp. Med. 195, 1549–1563.CrossRefPubMedGoogle Scholar
  4. 4.
    Zuk, P. A., Zhu, M., Ashjian, P., De Ugarte, D. A., Huang, J. I., Mizuno, H., et al. (2002) Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell 13, 4279–4295.CrossRefPubMedGoogle Scholar
  5. 5.
    Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., et al. (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7, 211–228.CrossRefPubMedGoogle Scholar
  6. 6.
    Gronthos, S., Zannettino, A. C., Hay, S. J., Shi, S., Graves, S. E., Kortesidis, A., et al. (2003) Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J. Cell Sci. 116, 1827–1835.CrossRefPubMedGoogle Scholar
  7. 7.
    Safford, K. M., Hicok, K. C., Safford, S. D., Halvorsen, Y. D., Wilkison, W. O., Gimble, J. M., et al. (2002) Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem. Biophys. Res. Commun. 294, 371–379.CrossRefPubMedGoogle Scholar
  8. 8.
    Woodbury, D., Reynolds, K., and Black, I. B. (2002) Adult bone marrow stromal stem cells express germline, ectodermal, endodermal, and mesodermal genes prior to neurogenesis. J. Neurosci. Res. 69, 908–917.CrossRefPubMedGoogle Scholar
  9. 9.
    Boquest A. C., Shahdadfar, A., Frønsdal, K., Sigurjonsson, O., Tunheim, S. H., Collas, P., et al. (2005) Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression following in vitro cell culture. Mol. Biol. Cell. 16, 1131–1141.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Andrew C. Boquest
    • 1
  • Aboulghassem Shahdadfar
    • 2
  • Jan E. Brinchmann
    • 2
  • Philippe Collas
    • 1
  1. 1.Institute of Medical BiochemistryUniversity of OsloOsloNorway
  2. 2.Institute of Immunology Rikshospitalet University Hospital and University of OsloRikshospitalet, OsloNorway

Personalised recommendations