Differential Display Methods and Protocols pp 267-277

Part of the Methods in Molecular Biology book series (MIMB, volume 317)

Identification of Target Genes of a Yeast Transcriptional Repressor

  • Bernard Mai
  • Linda L. Breeden

Abstract

Xbp1 is a transcriptional repressor with homology to the DNA binding domains of two cell-cycle regulatory transcription factors, Swi4 and Mbp1. Target genes bound and regulated by Xbp1, have been identified using differential display (DD). Because little was known about the regulation or function of Xbp1, we identified genes that were underrepresented in RNAs purified from cells in which Xbp1 was ectopically expressed. Four target genes, including three cyclin genes, were identified by DD and then confirmed by binding studies and Northern analysis. The binding site for Xbp1, previously identified by site selection, was present in these target genes and is conserved phylogenetically.

Key Words

Xbp1 yeast cyclins transcription differential meiosis 

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Bernard Mai
    • 1
  • Linda L. Breeden
    • 2
  1. 1.Research Scientist, Genomic SciencesSanofi-Aventis GroupFrankfurtGermany
  2. 2.Division of Basic SciencesFred Hutchinson Cancer Research CenterSeattle

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