Abstract
Small interfering RNA (siRNA) is a potent and specific method of inducing gene silencing through induction of RNA interference. siRNAs can be allowed for in vitro and in vivo applications. siRNAs have been successfully studied in vitro, but little is known about its efficacy in vivo. We have successfully applied the siRNA technique for silencing glucose transporter 3 in cultured L6 muscle cells. The siRNA technique has been used efficiently to silence RasGRP4 expression in mast cells.
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Acknowledgments
The authors thank Rhesa Dykes for technical assistance. This work was supported by Takeda Pharmaceuticals, ETSU grants 2-25453 and 2-25491.
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© 2006 Humana Press Inc.
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Yin, D., Stuart, C.A. (2006). Gene Silencing Using Small Interference RNA in Mast Cells. In: Krishnaswamy, G., Chi, D.S. (eds) Mast Cells. Methods in Molecular Biology, vol 315. Humana Press. https://doi.org/10.1385/1-59259-967-2:333
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DOI: https://doi.org/10.1385/1-59259-967-2:333
Publisher Name: Humana Press
Print ISBN: 978-1-58829-374-9
Online ISBN: 978-1-59259-967-7
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