Plant Cell Culture Protocols pp 357-368

Part of the Methods in Molecular Biology™ book series (MIMB, volume 318)

Methods for Regeneration and Transformation in Eschscholzia californica

A Model Plant to Investigate Alkaloid Biosynthesis
  • Benjamin P. MacLeod
  • Peter J. Facchini

Abstract

Eschscholzia californica Cham. (California poppy) is a plant species that accumulates pharmacologically active alkaloids biosynthetically related to the morphinan alkaloids of Papaver somniferum. This, in combination with the relative ease with which it is propagated in vitro, makes it a key model for benzylisoquinoline biosynthesis. Transformation techniques are an important tool for these studies and for metabolic engineering attempts. Agrobacterium mediated transformation techniques for this model species have been developed in our lab and used for modulation of transcript levels relevant to the biosynthesis of these alkaloids. Here we describe the techniques used in our lab for production of transgenic callus, hairy root cultures, and whole plants.

Key Words

Agrobacterium rhizogenes Agrobacterium tumefaciens benzylisoquinoline alkaloids California poppy Eschscholzia californica hairy roots plant transformation protocol somatic embryogenesis transgenic callus 

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Benjamin P. MacLeod
    • 1
  • Peter J. Facchini
    • 1
  1. 1.Department of Biological SciencesUniversity of CalgaryCalgary, AlbertaCanada

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