Yeast Protocol pp 107-120 | Cite as

Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

  • R. Daniel Gietz
  • Robin A. Woods
Part of the Methods in Molecular Biology book series (MIMB, volume 313)

Abstract

The technique for the transformation of Saccharomyces cerevisiae using the LiAc/SS Carrier DNA/PEG method is described. We describe a rapid method, for use when large numbers of transformants are not necessary. A high-efficiency method for the generation of large numbers of transformants is also given. A method for the transformation of plasmid libraries, which includes yeast two-hybrid applications, also is listed to aid the reader in generating transformants to effectively cover the library complexity. Finally, a protocol for transformation using a 96-well format is included for transformation applications that require it.

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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • R. Daniel Gietz
    • 1
  • Robin A. Woods
    • 2
  1. 1.Department of Biochemistry and Medical GenmeticsUniversity of ManitobaWinnipegCanada
  2. 2.Department of BiologyUniversity of WinnipegWinnipeg, ManitobaCanada

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