Yeast Protocol

Volume 313 of the series Methods in Molecular Biology pp 107-120

Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

  • R. Daniel GietzAffiliated withDepartment of Biochemistry and Medical Genmetics, University of Manitoba
  • , Robin A. WoodsAffiliated withDepartment of Biology, University of Winnipeg

* Final gross prices may vary according to local VAT.

Get Access


The technique for the transformation of Saccharomyces cerevisiae using the LiAc/SS Carrier DNA/PEG method is described. We describe a rapid method, for use when large numbers of transformants are not necessary. A high-efficiency method for the generation of large numbers of transformants is also given. A method for the transformation of plasmid libraries, which includes yeast two-hybrid applications, also is listed to aid the reader in generating transformants to effectively cover the library complexity. Finally, a protocol for transformation using a 96-well format is included for transformation applications that require it.