Isolation of Yeast Mitochondria

  • Chris Meisinger
  • Nikolaus Pfanner
  • Kaye N. Truscott
Part of the Methods in Molecular Biology book series (MIMB, volume 313)


Often preparations of isolated organelles contain other, unwanted, cellular components. For biochemical experiments to determine the localization of newly identified proteins, or to determine the whole set of proteins (or the proteome) from a desired organelle, these unwanted components often confuse the resulting data. For these types of studies, it is crucial to have highly pure fractions of the desired organelle. Here we describe a protocol for purification of mitochondria from Saccharomyces cerevisiae cells devoid of contamination from other cellular compartments.


Sucrose Gradient Homogenization Buffer Yeast Mitochondrion Resuspend Pellet Mitochondrial Sample 
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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Chris Meisinger
    • 1
  • Nikolaus Pfanner
    • 1
  • Kaye N. Truscott
    • 2
  1. 1.Institut für Biochemie und MolekularbiologieUniversität FreiburgFreiburgGermany
  2. 2.Department of BiochemistryLa Trobe UniversityMelbourneAustralia

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